C4c antibody | 10-12

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Mouse anti Human C4c

Product Type
Monoclonal Antibody
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2608 C E* F WB 0.1 mg
Mouse anti Human C4c antibody, clone 10-12 recognizes complement component 4c (C4c). The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b’s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. iC4b is further degraded into the inactive fragments C4d and C4c. C4c consists of two a-chain fragments, alpha 4 (13 kDa) and alpha 3 (25 kDa) linked to a beta chain through disulphide bridges and a gamma chain. It is expressed in the blood plasma.

Product Details

Target Species
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Purified human C4c.
Approx. Protein Concentrations

Storage Information

Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guaranteed until date of expiry. Please see product label.

More Information

P0C0L5 Related reagents
Entrez Gene
C4B Related reagents
For research purposes only

Applications of C4c antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Western Blotting
  1. 1Clone 10-12 binds both C4c and C4 and so is not suitable for all ELISA applications.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
For best results Bio-Rad recommend HISTAR detection kits.
Histology Positive Control Tissue
Kidney sections from post-streptococcal glomerulonephritis patients.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Product Specific References

References for C4c antibody

  1. Leung, E. et al. (2006) The complement regulator C4b-binding protein (C4BP) interacts with both the C4c and C4dg subfragments of the parent C4b ligand: evidence for synergy in C4BP subsite binding.
    Biochemistry. 45 (27): 8378-92.
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