c-Jun antibody | OTI2A9
Mouse anti c-Jun
c-Jun antibody recognizes c-Jun, also known as activator protein 1, enhancer-binding protein AP1, proto-oncogene c-Jun, or transcription factor AP-1.
c-Jun Antibody with Control Lysate
c-Jun antibody recognizes c-Jun, also known as activator protein 1, enhancer-binding protein AP1, proto-oncogene c-Jun, or transcription factor AP-1.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI2A9
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00740 | WB | 100 µl | ||||
| VMA00740KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human c-Jun antibody recognizes c-Jun, also known as Jun activation domain binding protein, activator protein 1, enhancer-binding protein AP1, proto-oncogene c-Jun and transcription factor AP-1.
JUN is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. JUN is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies (provided by RefSeq, Jul 2008).
Mouse anti Human c-Jun antibody detects bands of 43 and 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
JUN is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. JUN is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies (provided by RefSeq, Jul 2008).
Mouse anti Human c-Jun antibody detects bands of 43 and 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti c-Jun detects bands of approximately 43 & 48 kDa in HeLa cell lysates
- Product Form
- Purified IgG - Liquid
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein A from ascites
- Preparation
- 20μl Mouse monoclonal antibody affinity purified on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin - Immunogen
- E. coli-derived full length recombinant protein of human c-Jun
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P05412 Related reagents
- Entrez Gene
- JUN Related reagents
- GO Terms
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003702 RNA polymerase II transcription factor activity
- GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
- GO:0003713 transcription coactivator activity
- GO:0005100 Rho GTPase activator activity
- GO:0007179 transforming growth factor beta receptor signaling pathway
- GO:0007184 SMAD protein import into nucleus
- GO:0008063 Toll signaling pathway
- GO:0008134 transcription factor binding
- GO:0010843 promoter binding
- GO:0016563 transcription activator activity
- GO:0016564 transcription repressor activity
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0043392 negative regulation of DNA binding
- GO:0043922 negative regulation by host of viral transcription
- GO:0043923 positive regulation by host of viral transcription
- GO:0045087 innate immune response
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0051090 regulation of transcription factor activity
- GO:0051403 stress-activated MAPK cascade
- GO:0060395 SMAD protein signal transduction
- GO:0070412 R-SMAD binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of c-Jun antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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