c-Jun antibody | OTI2A9

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Mouse anti c-Jun

c-Jun antibody recognizes c-Jun, also known as activator protein 1, enhancer-binding protein AP1, proto-oncogene c-Jun, or transcription factor AP-1.

c-Jun Antibody with Control Lysate

c-Jun antibody recognizes c-Jun, also known as activator protein 1, enhancer-binding protein AP1, proto-oncogene c-Jun, or transcription factor AP-1.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI2A9
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00740 WB 100 µl
VMA00740KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human c-Jun antibody recognizes c-Jun, also known as Jun activation domain binding protein, activator protein 1, enhancer-binding protein AP1, proto-oncogene c-Jun and transcription factor AP-1.

JUN is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. JUN is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies (provided by RefSeq, Jul 2008).

Mouse anti Human c-Jun antibody detects bands of 43 and 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - Liquid
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein A from ascites
Preparation
20μl Mouse monoclonal antibody affinity purified on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen
E. coli-derived full length recombinant protein of human c-Jun

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P05412 Related reagents
Entrez Gene
JUN Related reagents
GO Terms
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003702 RNA polymerase II transcription factor activity
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0003713 transcription coactivator activity
GO:0005100 Rho GTPase activator activity
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007184 SMAD protein import into nucleus
GO:0008063 Toll signaling pathway
GO:0008134 transcription factor binding
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0043392 negative regulation of DNA binding
GO:0043922 negative regulation by host of viral transcription
GO:0043923 positive regulation by host of viral transcription
GO:0045087 innate immune response
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0051090 regulation of transcription factor activity
GO:0051403 stress-activated MAPK cascade
GO:0060395 SMAD protein signal transduction
GO:0070412 R-SMAD binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of c-Jun antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Mouse anti c-Jun detects bands of approximately 43 & 48 kDa in HeLa cell lysates
Western Blotting
Mouse anti c-Jun detects bands of approximately 43 & 48 kDa in HeLa cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB