c-Jun antibody | OTI2A9

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Anti c-Jun Antibody, clone OTI2A9 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with c-Jun antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 37 second exposure. Arrow points to c-Jun (molecular weight 43 & 48 kDa)

Mouse anti c-Jun

c-Jun antibody recognizes c-Jun, also known as activator protein 1, enhancer-binding protein AP1, proto-oncogene c-Jun, or transcription factor AP-1.

c-Jun Antibody with Control Lysate

c-Jun antibody recognizes c-Jun, also known as activator protein 1, enhancer-binding protein AP1, proto-oncogene c-Jun, or transcription factor AP-1.

Product Type: PrecisionAb™ Monoclonal
Clone: OTI2A9
Isotype: IgG1

Product Code	Applications	Pack Size
VMA00740	WB	100 µl
VMA00740KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human c-Jun antibody recognizes c-Jun, also known as Jun activation domain binding protein, activator protein 1, enhancer-binding protein AP1, proto-oncogene c-Jun and transcription factor AP-1.

JUN is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. JUN is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies (provided by RefSeq, Jul 2008).

Mouse anti Human c-Jun antibody detects bands of 43 and 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Product Form: Purified IgG - Liquid
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein A from ascites
Preparation: 20μl Mouse monoclonal antibody affinity purified on Protein A from ascites
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen: E. coli-derived full length recombinant protein of human c-Jun

Storage Information

Storage: Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch
Shelf Life: As supplied, 12 months from date of despatch

More Information

UniProt: P05412 Related reagents
Entrez Gene: JUN Related reagents
GO Terms: GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0003702 RNA polymerase II transcription factor activity; GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity; GO:0003713 transcription coactivator activity; GO:0005100 Rho GTPase activator activity; GO:0007179 transforming growth factor beta receptor signaling pathway; GO:0007184 SMAD protein import into nucleus; GO:0008063 Toll signaling pathway; GO:0008134 transcription factor binding; GO:0010843 promoter binding; GO:0016563 transcription activator activity; GO:0016564 transcription repressor activity; GO:0034130 toll-like receptor 1 signaling pathway; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0043392 negative regulation of DNA binding; GO:0043922 negative regulation by host of viral transcription; GO:0043923 positive regulation by host of viral transcription; GO:0045087 innate immune response; GO:0045944 positive regulation of transcription from RNA polymerase II promoter; GO:0051090 regulation of transcription factor activity; GO:0051403 stress-activated MAPK cascade; GO:0060395 SMAD protein signal transduction; GO:0070412 R-SMAD binding
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of c-Jun antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000
Western Blotting			1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Western Blotting: Mouse anti c-Jun detects bands of approximately 43 & 48 kDa in HeLa cell lysates
Western Blotting: Mouse anti c-Jun detects bands of approximately 43 & 48 kDa in HeLa cell lysates
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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