BUB1B antibody | OTI5D9

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Anti BUB1B Antibody, clone OTI5D9 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti BUB1B Antibody, clone OTI5D9 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti BUB1B Antibody, clone OTI5D9 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human BUB1B antibody, clone OTI5D9 (**VMA00256**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to BUB1B (molecular weight 130 kDa).

Anti BUB1B Antibody, clone OTI5D9 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of BUB1B from HeLa whole cell lysate. The Input lane is 10% input; the BUB1B-IP lane is the immunoprecipitation with the BUB1B antibody (2.5 µg, **VMA00256**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using BUB1B antibody (1/1,000, **VMA00256**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to BUB1B.

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Mouse anti BUB1B

Product Type: PrecisionAb Monoclonal
Clone: OTI5D9
Isotype: IgG1
Format: Purified
Specificity: BUB1B

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Mouse anti Human BUB1B antibody, clone OTI5D9 recognizes the mitotic checkpoint serine/threonine-protein kinase BUB1 beta, also known as MAD3/BUB1-related protein kinase, budding uninhibited by benzimidazoles 1 homolog beta, mitotic checkpoint kinase MAD3L and mitotic checkpoint serine/threonine-protein kinase BUB1 beta.

The BUB1B gene encodes a kinase involved in spindle checkpoint function. This protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer (provided by RefSeq, Jul 2008).

Mouse anti Human BUB1B antibody, clone OTI5D9 detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti BUB1B detects a band of approximately 130 kDa in HeLa cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full-length protein expressed in 293T cell transfected with human BUB1B expression vector
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.