BUB1B antibody | OTI5D9

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Mouse anti BUB1B

Product Type
PrecisionAb Monoclonal
Clone
OTI5D9
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00256
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human BUB1B antibody recognizes the mitotic checkpoint serine/threonine-protein kinase BUB1 beta, also known as MAD3/BUB1-related protein kinase, budding uninhibited by benzimidazoles 1 homolog beta, mitotic checkpoint kinase MAD3L and mitotic checkpoint serine/threonine-protein kinase BUB1 beta.

The BUB1B gene encodes a kinase involved in spindle checkpoint function. This protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer (provided by RefSeq, Jul 2008).

Mouse anti Human BUB1B antibody detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti BUB1B detects a band of approximately 130 kDa in HeLa cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full-length protein expressed in 293T cell transfected with human BUB1B expression vector

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
O60566 Related reagents
Entrez Gene
BUB1B Related reagents
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of BUB1B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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