BUB1B antibody | OTI5D9

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Mouse anti BUB1B

BUB1B antibody recognizes the mitotic checkpoint serine/threonine-protein kinase BUB1 beta, also known as MAD3/BUB1-related protein kinase, budding uninhibited by benzimidazoles 1 homolog beta or mitotic checkpoint kinase MAD3L.

BUB1B Antibody with Control Lysate

BUB1B antibody recognizes the mitotic checkpoint serine/threonine-protein kinase BUB1 beta, also known as MAD3/BUB1-related protein kinase, budding uninhibited by benzimidazoles 1 homolog beta or mitotic checkpoint kinase MAD3L.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI5D9
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00256 WB 100 µl
VMA00256KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human BUB1B antibody recognizes the mitotic checkpoint serine/threonine-protein kinase BUB1 beta, also known as MAD3/BUB1-related protein kinase, budding uninhibited by benzimidazoles 1 homolog beta, mitotic checkpoint kinase MAD3L and mitotic checkpoint serine/threonine-protein kinase BUB1 beta.

The BUB1B gene encodes a kinase involved in spindle checkpoint function. This protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer (provided by RefSeq, Jul 2008).

Mouse anti Human BUB1B antibody detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full-length protein expressed in 293T cell transfected with human BUB1B expression vector

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
O60566 Related reagents
Entrez Gene
BUB1B Related reagents
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of BUB1B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti BUB1B detects a band of approximately 130 kDa in HeLa cell lysates
Western Blotting
Anti BUB1B detects a band of approximately 130 kDa in HeLa cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB