BUB1 antibody | 14H5

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Mouse anti Human BUB1

Product Type
Monoclonal Antibody
Clone
14H5
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2679 IF* IP WB 50 µg
Mouse anti Human BUB1 antibody, clone 14H5 detects Bub1, a protein kinase involved in spindle checkpoint function during mitosis. Bub1 co-operates with Aurora B kinase to inhibit Cdc20-mediated activation of the anaphase promoting complex/cyclosome (APC/C) until all the chromosomes correctly align on the microtubule spindle apparatus via their kinetochores, thus ensuring high fidelity mitotic chromosome segregation.

Bub1 is also required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshin protein, and metaphase congression. Mutation or aberrant Bub1 expression is associated with chromosomal instability, aneuploidy, and human cancer.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Sodium Azide (NaN3)
Immunogen
Recombinant protein corresponding to amino acids 281-419 of human Bub1 protein.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O43683 Related reagents
Entrez Gene
BUB1 Related reagents
GO Terms
GO:0007059 chromosome segregation
GO:0005515 protein binding
GO:0000236 mitotic prometaphase
GO:0000777 condensed chromosome kinetochore
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0007063 regulation of sister chromatid cohesion
GO:0007094 mitotic cell cycle spindle assembly checkpoint
GO:0044419 interspecies interaction between organisms
GO:0051301 cell division
Regulatory
For research purposes only

Applications of BUB1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1 1/500 1/2000
Immunoprecipitation 1/200
Western Blotting 1/500 1/2000
  1. 1Use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH6.8, or 100% methanol at
    -20oC. Permeabilise cells with 0.5% Triron X-100.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2679 detects a band of approximately 160kDa in HeLa cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

Further Reading

  1. Morrow, C.J. et al. (2005) Bub1 and aurora B cooperate to maintain BubR1-mediated inhibition of APC/CCdc20.
    J Cell Sci. 118 (Pt 16): 3639-52.
  2. Meraldi, P. & Sorger, P.K. (2005) A dual role for Bub1 in the spindle checkpoint and chromosome congression.
    EMBO J. 24 (8): 1621-33.
  3. Vanoosthuyse, V. & Hardwick, K.G. (2005) Bub1 and the multilayered inhibition of Cdc20-APC/C in mitosis.
    Trends Cell Biol. 15 (5): 231-3.