BTK antibody | OTI12D4

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Anti BTK Antibody, clone OTI12D4 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti BTK Antibody, clone OTI12D4 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti BTK Antibody, clone OTI12D4 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human BTK antibody, clone OTI12D4 (**VMA00176**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to BTK (molecular weight 72 kDa).

Anti BTK Antibody, clone OTI12D4 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of BTK from K562 whole cell lysate. The Input lane is 10% input; the BTK-IP lane is the immunoprecipitation with the BTK antibody (2.5 µg, **VMA00176**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA929). Western blot analysis was performed using BTK antibody (1/2,000, **VMA00176**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to BTK.

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Mouse anti BTK

Product Type: PrecisionAb Monoclonal
Clone: OTI12D4
Isotype: IgG2a
Format: Purified
Specificity: BTK

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00176 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human BTK antibody, clone OTI12D4 recognizes BTK, also known as B-cell progenitor kinase, gamma globulinaemia tyrosine kinase, dominant-negative kinase-deficient Brutons tyrosine kinase, truncated Bruton gamma globulinemia tyrosine kinase, tyrosine-protein kinase BTK and tyrosine-protein kinase BTK isoform (lacking exon 14).

The protein encoded by the BTK gene plays a crucial role in B-cell development. Mutations in BTK cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement (provided by RefSeq, Nov 2008).

Mouse anti Human BTK antibody, clone OTI12D4 detects a band of 72 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti BTK detects a band of approximately 72 kDa in K562 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human BTK (NP_000052) produced in HEK293T cells
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q06187
Entrez Gene: BTK
GO Terms: GO:0005886 plasma membrane; GO:0005829 cytosol; GO:0004715 non-membrane spanning protein tyrosine kinase activity; GO:0046872 metal ion binding; GO:0005524 ATP binding; GO:0005547 phosphatidylinositol-3,4,5-trisphosphate binding; GO:0005634 nucleus; GO:0019722 calcium-mediated signaling; GO:0007498 mesoderm development; View More GO Terms; GO:0008624 induction of apoptosis by extracellular signals; GO:0042802 identical protein binding; GO:0045121 membrane raft