BTK antibody | OTI12D4

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Anti BTK Antibody, clone OTI12D4 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with BTK antibody (**VMA00176**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 8 second exposure. Arrow points to BTK (molecular weight 72 kDa).

Mouse anti BTK

BTK antibody recognizes the tyrosine kinase BTK, also known as B-cell progenitor kinase or Bruton's agammaglobulinaemia tyrosine kinase. This antibody has been extensively validated for western blotting using whole cell lysates.

BTK Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: OTI12D4
Isotype: IgG2a
Format: Purified

Product Code	Applications	Pack Size
VMA00176	WB	100 µl
VMA00176KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human BTK antibody recognizes BTK, also known as B-cell progenitor kinase, gamma globulinaemia tyrosine kinase, dominant-negative kinase-deficient Brutons tyrosine kinase, truncated Bruton gamma globulinemia tyrosine kinase, tyrosine-protein kinase BTK and tyrosine-protein kinase BTK isoform (lacking exon 14).

The protein encoded by the BTK gene plays a crucial role in B-cell development. Mutations in BTK cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement (provided by RefSeq, Nov 2008).

Mouse anti Human BTK antibody detects a band of 72 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti BTK detects a band of approximately 72 kDa in K562 cell lysates.
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human BTK (NP_000052) produced in HEK293T cells
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg K562 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: Q06187 Related reagents
Entrez Gene: BTK Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0005829 cytosol; GO:0004715 non-membrane spanning protein tyrosine kinase activity; GO:0046872 metal ion binding; GO:0005524 ATP binding; GO:0005547 phosphatidylinositol-3,4,5-trisphosphate binding; GO:0005634 nucleus; GO:0019722 calcium-mediated signaling; GO:0007498 mesoderm development; GO:0008624 induction of apoptosis by extracellular signals; GO:0042802 identical protein binding; GO:0045121 membrane raft
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of BTK antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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