BTK antibody | OTI12D4
Mouse anti BTK
BTK antibody recognizes the tyrosine kinase BTK, also known as B-cell progenitor kinase or Bruton's agammaglobulinaemia tyrosine kinase. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI12D4
- Isotype
- IgG2a
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00176 | WB | 100 µl |
Mouse anti Human BTK antibody recognizes BTK, also known as B-cell progenitor kinase, gamma globulinaemia tyrosine kinase, dominant-negative kinase-deficient Brutons tyrosine kinase, truncated Bruton gamma globulinemia tyrosine kinase, tyrosine-protein kinase BTK and tyrosine-protein kinase BTK isoform (lacking exon 14).
The protein encoded by the BTK gene plays a crucial role in B-cell development. Mutations in BTK cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement (provided by RefSeq, Nov 2008).
Mouse anti Human BTK antibody detects a band of 72 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by the BTK gene plays a crucial role in B-cell development. Mutations in BTK cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement (provided by RefSeq, Nov 2008).
Mouse anti Human BTK antibody detects a band of 72 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti BTK detects a band of approximately 72 kDa in K562 cell lysates.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human BTK (NP_000052) produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q06187 Related reagents
- Entrez Gene
- BTK Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0005829 cytosol
- GO:0004715 non-membrane spanning protein tyrosine kinase activity
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0005547 phosphatidylinositol-3,4,5-trisphosphate binding
- GO:0005634 nucleus
- GO:0019722 calcium-mediated signaling
- GO:0007498 mesoderm development
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0042802 identical protein binding
- GO:0045121 membrane raft
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of BTK antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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