BRCA1 antibody | 6B4

Mouse anti BRCA1

Product Type
Monoclonal Antibody
Clone
6B4
Isotype
IgG1
Specificity
BRCA1

Product Code Applications Pack Size List Price Your Price Qty
MCA5945GT
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SDS Safety Datasheet SDS
IF IP P WB 50 µg loader
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Mouse anti BRCA1 antibody, clone 6B4 recognizes both human and mouse BRCA1, otherwise known as breast cancer type 1 susceptibility protein, a tumor suppressor gene and major player in DNA damage repair, predominantly expressed in the nucleus during the S/G2 phase of the cell cycle.

Along with BRCA2, BRCA1 is a high risk gene which is associated with hereditary breast and ovarian cancers, particularly at a younger age of diagnosis. Women carrying the BRCA1 mutation have a 50-95% chance of developing breast cancer in later life, but genetic screening and increased awareness of preventative surgery can reduce this risk significantly (Ford et al. 1994). Deleterious BRCA1 mutations may also increase the risk of other cancers in both males and females including pancreatic cancer, although in males pancreatic and prostate cancer appear to be more strongly associated with BRCA2 gene mutations.

BRCA1 is a key marker of triple-negative breast cancer/TNBC (ER-/PR-/HER2-), a high risk aggressive cancer which makes up about 15% of invasive breast cancers, and which lacks the benefit of specific therapy that targets the three major proteins ER/PR/HER2. Triple-negative tumors are predominantly basal-like, poorly differentiated and of higher histological grade. Younger women have an increased rate of basal or BRCA related TNBC, compared with the higher proportion of apocrine, normal-like and rare subtypes of TNBC, seen in older women.

Mouse anti BRCA1, clone 6B4 recognizes the full length ~220 kDa BRCA1, but does not recognize the delta11b splice variant. The breast milk cell line HBL100 can be used as a positive control.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E.coli expressed BRCA1 protein fragment corresponding to amino acids 341-748.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/400
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting 1/250 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Breast carcinoma

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References for BRCA1 antibody

  1. Scully, R. et al. (1996) Location of BRCA1 in human breast and ovarian cancer cells.
    Science. 272 (5258): 123-6.
  2. Chen, Y. et al. (1996) Response: Location of BRCA1 in Human Breast and Ovarian Cancer Cells.
    Science. 272 (5258): 125-6.
  3. Zhong, Q. et al. (1999) Association of BRCA1 with the hRad50-hMre11-p95 complex and the DNA damage response.
    Science. 285 (5428): 747-50.

Synonyms
Breast Cancer Type 1 Susceptibility Protein
UniProt
P38398
P48754
Entrez Gene
BRCA1
Brca1
GO Terms
GO:0007059 chromosome segregation
GO:0005515 protein binding
GO:0000724 double-strand break repair via homologous recombination
GO:0003677 DNA binding
GO:0003713 transcription coactivator activity
GO:0003723 RNA binding
GO:0030529 ribonucleoprotein complex
GO:0006915 apoptosis
GO:0004842 ubiquitin-protein ligase activity
GO:0005654 nucleoplasm
GO:0006301 postreplication repair
GO:0006359 regulation of transcription from RNA polymerase III promoter
GO:0006633 fatty acid biosynthetic process
GO:0006978 DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator
GO:0007049 cell cycle
GO:0008270 zinc ion binding
GO:0008274 gamma-tubulin ring complex
GO:0008630 DNA damage response, signal transduction resulting in induction of apoptosis
GO:0042802 identical protein binding
GO:0010212 response to ionizing radiation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0015631 tubulin binding
GO:0016481 negative regulation of transcription
GO:0016563 transcription activator activity
GO:0030521 androgen receptor signaling pathway
GO:0031398 positive regulation of protein ubiquitination
GO:0031436 BRCA1-BARD1 complex
GO:0031572 G2/M transition DNA damage checkpoint
GO:0031625 ubiquitin protein ligase binding
GO:0042127 regulation of cell proliferation
GO:0043627 response to estrogen stimulus
GO:0045717 negative regulation of fatty acid biosynthetic process
GO:0045739 positive regulation of DNA repair
GO:0046600 negative regulation of centriole replication
GO:0050681 androgen receptor binding
GO:0051865 protein autoubiquitination
GO:0070531 BRCA1-A complex
GO:0071681 cellular response to indole-3-methanol
GO:0085020 protein K6-linked ubiquitination
GO:0006260 DNA replication
GO:0000794 condensed nuclear chromosome
GO:0003684 damaged DNA binding
GO:0005813 centrosome
GO:0006281 DNA repair
GO:0006310 DNA recombination
GO:0009048 dosage compensation, by inactivation of X chromosome
GO:0043009 chordate embryonic development
GO:0051298 centrosome duplication
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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