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Braf antibody | OTI4B2

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Anti Braf Antibody, clone OTI4B2 (PrecisionAb Monoclonal Antibody) thumbnail image 1
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Western blot analysis of whole cell lysates probed with BRAF antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to BRAF (molecular weight 86 kDa)

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WB

Mouse anti Braf

Product Type
PrecisionAb Monoclonal
Clone
OTI4B2
Isotype
IgG2a
Format
Purified
Specificity
Braf

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VMA00632
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WB 100 µl loader
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Mouse anti Human BRAF antibody recognizes the serine/threonine-protein kinase B-raf, also known as 94 kDa B-raf protein, B-Raf proto-oncogene serine/threonine-protein kinase (p94), murine sarcoma viral (v-raf) oncogene homolog B1, proto-oncogene B-Raf, serine/threonine-protein kinase B-raf, v-raf murine sarcoma viral oncogene homolog B and v-raf murine sarcoma viral oncogene homolog B1.

BRAF encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in BRAF are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in BRAF have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for BRAF (provided by RefSeq, Jul 2008).

Mouse anti BRAF detects a band of 86 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti BRAF antibody detects a band of approximately 86 kDa in C2C12 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
HEK293-derived full length recombinant BRAF
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P15056
Entrez Gene
BRAF
GO Terms
GO:0005886 plasma membrane
GO:0000186 activation of MAPKK activity
GO:0005829 cytosol
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006916 anti-apoptosis
GO:0007264 small GTPase mediated signal transduction
GO:0007268 synaptic transmission
GO:0009887 organ morphogenesis
GO:0048011 nerve growth factor receptor signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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