Braf antibody | OTI4B2

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Anti Braf Antibody, clone OTI4B2 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Braf Antibody, clone OTI4B2 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with BRAF antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to BRAF (molecular weight 86 kDa)

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Mouse anti Braf

Product Type: PrecisionAb Monoclonal
Clone: OTI4B2
Isotype: IgG2a
Format: Purified
Specificity: Braf

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VMA00632 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human BRAF antibody recognizes the serine/threonine-protein kinase B-raf, also known as 94 kDa B-raf protein, B-Raf proto-oncogene serine/threonine-protein kinase (p94), murine sarcoma viral (v-raf) oncogene homolog B1, proto-oncogene B-Raf, serine/threonine-protein kinase B-raf, v-raf murine sarcoma viral oncogene homolog B and v-raf murine sarcoma viral oncogene homolog B1.

BRAF encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in BRAF are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in BRAF have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for BRAF (provided by RefSeq, Jul 2008).

Mouse anti BRAF detects a band of 86 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Mouse anti BRAF antibody detects a band of approximately 86 kDa in C2C12 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen: HEK293-derived full length recombinant BRAF
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P15056
Entrez Gene: BRAF
GO Terms: GO:0005886 plasma membrane; GO:0000186 activation of MAPKK activity; GO:0005829 cytosol; GO:0046872 metal ion binding; GO:0005524 ATP binding; GO:0005634 nucleus; GO:0006916 anti-apoptosis; GO:0007264 small GTPase mediated signal transduction; GO:0007268 synaptic transmission; View More GO Terms; GO:0009887 organ morphogenesis; GO:0048011 nerve growth factor receptor signaling pathway