Braf antibody | OTI4B2
Mouse anti Braf
BRAF antibody recognizes the serine/threonine-protein kinase B-raf, also known as 94 kDa B-raf protein, B-Raf proto-oncogene serine/threonine-protein kinase (p94), murine sarcoma viral (v-raf) oncogene homolog B1, proto-oncogene B-Raf, or v-raf murine sarcoma viral oncogene homolog B.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI4B2
- Isotype
- IgG2a
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00632 | WB | 100 µl |
Mouse anti Human BRAF antibody recognizes the serine/threonine-protein kinase B-raf, also known as 94 kDa B-raf protein, B-Raf proto-oncogene serine/threonine-protein kinase (p94), murine sarcoma viral (v-raf) oncogene homolog B1, proto-oncogene B-Raf, serine/threonine-protein kinase B-raf, v-raf murine sarcoma viral oncogene homolog B and v-raf murine sarcoma viral oncogene homolog B1.
BRAF encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in BRAF are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in BRAF have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for BRAF (provided by RefSeq, Jul 2008).
Mouse anti BRAF detects a band of 86 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
BRAF encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in BRAF are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in BRAF have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for BRAF (provided by RefSeq, Jul 2008).
Mouse anti BRAF detects a band of 86 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti BRAF antibody detects a band of approximately 86 kDa in C2C12 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol - Immunogen
- HEK293-derived full length recombinant BRAF
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P15056 Related reagents
- Entrez Gene
- BRAF Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0000186 activation of MAPKK activity
- GO:0005829 cytosol
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0006916 anti-apoptosis
- GO:0007264 small GTPase mediated signal transduction
- GO:0007268 synaptic transmission
- GO:0009887 organ morphogenesis
- GO:0048011 nerve growth factor receptor signaling pathway
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Braf antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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