BOB-1 antibody | RM378

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Rabbit anti BOB-1

Product Type
Monoclonal Antibody
Clone
RM378
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6355
Datasheet
P WB
SDS
0.1 ml loader

Rabbit anti BOB-1 antibody recognizes POU domain class 2-associating factor 1 (POU2AF1), also known as B-cell-specific coactivator OBF-1 and BOB-1.

BOB-1 is a lymphocyte-specific transcriptional regulator which interacts with transcription factors Oct1 and Oct2 to enhance octamer-dependent transcription (Levels et al. 2019). BOB-1 expression depends on lymphocyte type, B cells show constitutive expression whereas expression is inducible in T cells following costimulation. Mice without BOB-1 lack germinal centres and have a range of defects in B-cell development (Boehm et al. 2001). BOB1 is used to differentiate classical Hodgkin's lymphoma (BOB1 negative) from primary mediastinal large B-cell lymphoma (positive) (Hoeller et al. 2010).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to the C-terminus of human BOB-1

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q16633
Entrez Gene
POU2AF1
GO Terms
GO:0005515 protein binding
GO:0003713 transcription coactivator activity
GO:0005634 nucleus
GO:0006366 transcription from RNA polymerase II promoter
GO:0006959 humoral immune response
Regulatory
For research purposes only

Applications of BOB-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/1000 1/2000
Western Blotting 1/5000 1/10000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

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