Rabbit anti BOB-1 antibody
recognizes POU domain class 2-associating factor 1 (POU2AF1), also known as B-cell-specific coactivator OBF-1 and BOB-1.
BOB-1 is a lymphocyte-specific transcriptional regulator which interacts with transcription factors Oct1 and Oct2 to enhance octamer-dependent transcription (Levels et al. 2019
). BOB-1 expression depends on lymphocyte type, B cells show constitutive expression whereas expression is inducible in T cells following costimulation. Mice without BOB-1 lack germinal centres and have a range of defects in B-cell development (Boehm et al. 2001
). BOB1 is used to differentiate classical Hodgkin's lymphoma (BOB1 negative) from primary mediastinal large B-cell lymphoma (positive) (Hoeller et al. 2010
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
- A peptide corresponding to the C-terminus of human BOB-1
- Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
transcription coactivator activity
transcription from RNA polymerase II promoter
humoral immune response
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of BOB-1 antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra