BID antibody | CD01/1H1

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Mouse anti BID

Product Type
PrecisionAb Monoclonal
Clone
CD01/1H1
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00816
Datasheet
WB
SDS
100 µl loader

Mouse anti BID antibody recognizes BH3-interacting domain death agonist.

BID is a member of the BCL2 family, and plays pro-apoptotic roles within the intrinsic apoptotic pathway. Cleavage of BID by caspase-8 leads to permeabilization of the mitochondrial membrane, a process that initiates the caspase cascade. Non-apoptotic roles of BID include modulation of inflammatory signaling in astrocytes and modulation of the immunological profile in microglia and macrophages (Kinsella et al. 2018). Because of its apoptotic role, the expression level of BID in cancer cells is critical to their death or survival. Silencing BID makes cancer cells resistant to apoptosis, and overexpression can sensitize some cancer cell lines to apoptosis induced by anticancer agents (Orzechowska et al. 2015). Mimicry of BID has also been identified as a mechanism by which viruses can evade host cells (Choi et al. 2012).

Product Details

Target Species
Human
Western Blotting
Mouse anti BID antibody detects a band of approximately 20 kDa in HEK293 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-195 of human BID
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P55957
Entrez Gene
BID
GO Terms
GO:0001836 release of cytochrome c from mitochondria
GO:0005829 cytosol
GO:0005123 death receptor binding
GO:0005624 membrane fraction
GO:0005741 mitochondrial outer membrane
GO:0008625 induction of apoptosis via death domain receptors
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0051402 neuron apoptosis
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of BID antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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