BETA-III Spectrin antibody

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Anti BETA-III Spectrin Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with beta-III spectrin antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to beta-III spectrin (molecular weight 270 kDa)

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Mouse anti BETA-III Spectrin

Product Type: PrecisionAb Monoclonal
Isotype: IgG2b
Format: Purified
Specificity: BETA-III Spectrin

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VMA00694 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human beta-III spectrin antibody recognizes the beta-III spectrin, also known as SCA5.

Spectrins are principle components of a cell's membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by SPTBN2 (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane. Mutations in SPTBN2 cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements (provided by RefSeq, Dec 2009).

Mouse anti Human beta-III spectrin antibody detects a band of 270 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Mouse anti beta-III spectrin detects a band of approximately 270 kDa in HepG2 cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: Recombinant protein of partial human beta-III spectrin (amino acids 356-475)
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: O15020
Entrez Gene: SPTBN2
GO Terms: GO:0003779 actin binding; GO:0005829 cytosol; GO:0005200 structural constituent of cytoskeleton; GO:0007411 axon guidance; GO:0008091 spectrin; GO:0008219 cell death; GO:0051693 actin filament capping