BETA-III Spectrin antibody
Spectrins are principle components of a cell's membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by SPTBN2 (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane. Mutations in SPTBN2 cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements (provided by RefSeq, Dec 2009).
Mouse anti Human beta-III spectrin antibody detects a band of 270 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Mouse anti beta-III spectrin detects a band of approximately 270 kDa in HepG2 cell lysates
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Mouse monoclonal antibody affinity purified on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Recombinant protein of partial human beta-III spectrin (amino acids 356-475)
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- As supplied, 12 months from date of despatch
- O15020 Related reagents
- Entrez Gene
- SPTBN2 Related reagents
- GO Terms
- GO:0003779 actin binding
- GO:0005829 cytosol
- GO:0005200 structural constituent of cytoskeleton
- GO:0007411 axon guidance
- GO:0008091 spectrin
- GO:0008219 cell death
- GO:0051693 actin filament capping
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- For research purposes only
Applications of BETA-III Spectrin antibody
|Application Name||Verified||Min Dilution||Max Dilution|
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Mouse IgG (H/L):HRP||STAR207P||WB||2 ml|
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