Beta Amyloid antibody | MAb1.1
Mouse anti Human beta-amyloid protein 42 antibody, clone Mab1.1 recognizes Beta-amyloid protein 42 (also known as Aβ42), the 42 amino acid peptide processed from Amyloid precursor protein (APP). There are several forms of Aβ peptides processed from APP, the most common being Aβ40 and Aβ42. Amyloid plaques contain both Aβ40 and Aβ42. Aβ40 is more common, but Aβ42 more fibrillogenic and therefore associated with disease states such as Alzheimer’s disease.
Mouse anti Human beta-amyloid protein 42 antibody, clone Mab1.1 specifically binds to the 37-42aa region, and does not recognise full-length APP or the 40 amino acid peptide processed from APP (Aβ40). It stains diffuse plaques found in the brain of Alzheimer’s disease patients. It does not stain healthy brain tissue or other organs.
Mouse anti Human beta-amyloid protein 42 antibody, clone Mab1.1 specifically binds to the 37-42aa region, and does not recognise full-length APP or the 40 amino acid peptide processed from APP (Aβ40). It stains diffuse plaques found in the brain of Alzheimer’s disease patients. It does not stain healthy brain tissue or other organs.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- A construct of the last six amino acids of Aβ42 (GGVVIA) and a potent T-cell epitope from Chlamydia pneumonia CWL pmp8-protein, synthesised directly onto an LPA scaffold (See Axelsen et al. for full details).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
Applications of Beta Amyloid antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.2 ug/ml | ||
| Immunohistology - Frozen | 20 | 40 ug/ml | |
| Immunohistology - Paraffin |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- If plates are coated with 2ug/ml Aβ42, clone Mab1.1 can be used at a dilution of 0.2ug/ml for an OD of approximately 2.
- Immunohistology
- This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Histology Positive Control Tissue
- Alzheimer's disease brain
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Secondary Antibodies Available
Product Specific References
References for Beta Amyloid antibody
-
Axelsen, T.V. et al. (2009) Specific recognition of the C-terminal end of A beta 42 by a high affinity monoclonal antibody.
Mol Immunol. 46 (11-12): 2267-73.
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