Beta Amyloid antibody | MAb1.1

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Mouse anti Human Beta Amyloid (aa37-42)

Product Type
Monoclonal Antibody
Clone
MAb1.1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5930GA C E P 0.1 mg
Mouse anti Human beta-amyloid protein 42 antibody, clone Mab1.1 recognizes Beta-amyloid protein 42 (also known as Aβ42), the 42 amino acid peptide processed from Amyloid precursor protein (APP). There are several forms of Aβ peptides processed from APP, the most common being Aβ40 and Aβ42. Amyloid plaques contain both Aβ40 and Aβ42. Aβ40 is more common, but Aβ42 more fibrillogenic and therefore associated with disease states such as Alzheimer’s disease.

Mouse anti Human beta-amyloid protein 42 antibody, clone Mab1.1 specifically binds to the 37-42aa region, and does not recognise full-length APP or the 40 amino acid peptide processed from APP (Aβ40). It stains diffuse plaques found in the brain of Alzheimer’s disease patients. It does not stain healthy brain tissue or other organs.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
A construct of the last six amino acids of Aβ42 (GGVVIA) and a potent T-cell epitope from Chlamydia pneumonia CWL pmp8-protein, synthesised directly onto an LPA scaffold (See Axelsen et al. for full details).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P05067 Related reagents
Entrez Gene
APP Related reagents
Regulatory
For research purposes only

Applications of Beta Amyloid antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 ug/ml
Immunohistology - Frozen 20 40 ug/ml
Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
If plates are coated with 2ug/ml Aβ42, clone Mab1.1 can be used at a dilution of 0.2ug/ml for an OD of approximately 2.
Immunohistology
This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Alzheimer's disease brain

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Beta Amyloid antibody

  1. Axelsen, T.V. et al. (2009) Specific recognition of the C-terminal end of A beta 42 by a high affinity monoclonal antibody.
    Mol Immunol. 46 (11-12): 2267-73.