Beta 2 Microglobulin antibody
Mouse anti Beta 2 Microglobulin
Beta 2 microglobulin antibody recognizes beta 2 microglobulin. The antibody has been extensively validated for western blotting using whole cell lysates.
Beta 2 Microglobulin Antibody with Control Lysate
Beta 2 microglobulin antibody recognizes beta 2 microglobulin. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00003 | WB | 100 µl | ||||
| VMA00003KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human beta 2 microglobulin antibody recognizes native human beta 2 microglobulin, a 119 amino acid component of MHC class I molecules present on all nucleated cells, containing a single Ig-like C1 type domain. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients (Woo et al. 2014). Also known as B2M, it has been suggested that this protein can be used as a prognostic marker for HIV infection (Halota et al. 2002).
Mouse anti Human beta 2 microglobulin antibody recognizes β2 microglobulin as a band of ~12 kDa in HeLa cell lysates.
Mouse anti Human beta 2 microglobulin antibody recognizes β2 microglobulin as a band of ~12 kDa in HeLa cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti beta 2 microglobulin antibody detects a band of approximately 12 kDa in HeLa cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
5% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
5% Glycerol - Immunogen
- Highly pure human beta 2 microglobulin from urine
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P61769 Related reagents
- Entrez Gene
- B2M Related reagents
- GO Terms
- GO:0000139 Golgi membrane
- GO:0005515 protein binding
- GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
- GO:0042612 MHC class I protein complex
- GO:0006955 immune response
- GO:0016032 viral reproduction
- GO:0031901 early endosome membrane
- GO:0050690 regulation of defense response to virus by virus
- GO:0060333 interferon-gamma-mediated signaling pathway
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Beta 2 Microglobulin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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