Beta 2 Microglobulin antibody

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Mouse anti Beta 2 Microglobulin

Beta 2 microglobulin antibody recognizes beta 2 microglobulin. The antibody has been extensively validated for western blotting using whole cell lysates.

Beta 2 Microglobulin Antibody with Control Lysate

Beta 2 microglobulin antibody recognizes beta 2 microglobulin. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00003 WB 100 µl
VMA00003KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human beta 2 microglobulin antibody recognizes native human beta 2 microglobulin, a 119 amino acid component of MHC class I molecules present on all nucleated cells, containing a single Ig-like C1 type domain. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients (Woo et al. 2014). Also known as B2M, it has been suggested that this protein can be used as a prognostic marker for HIV infection (Halota et al. 2002).

Mouse anti Human beta 2 microglobulin antibody recognizes β2 microglobulin as a band of ~12 kDa in HeLa cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
5% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
5% Glycerol
Immunogen
Highly pure human beta 2 microglobulin from urine

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P61769 Related reagents
Entrez Gene
B2M Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0005515 protein binding
GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
GO:0042612 MHC class I protein complex
GO:0006955 immune response
GO:0016032 viral reproduction
GO:0031901 early endosome membrane
GO:0050690 regulation of defense response to virus by virus
GO:0060333 interferon-gamma-mediated signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Beta 2 Microglobulin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti beta 2 microglobulin antibody detects a band of approximately 12 kDa in HeLa cell lysates.
Western Blotting
Anti beta 2 microglobulin antibody detects a band of approximately 12 kDa in HeLa cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB