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Beta 2 Microglobulin antibody

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Western Blot Validated
Anti Beta 2 Microglobulin Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with beta 2 microglobulin antibody (VMA00003) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 166 second exposure, Arrow points to beta 2 microglobulin (molecular weight 12 kDa).

Mouse anti Beta 2 Microglobulin

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00003
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human beta 2 microglobulin antibody recognizes native human beta 2 microglobulin, a 119 amino acid component of MHC class I molecules present on all nucleated cells, containing a single Ig-like C1 type domain. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients (Woo et al. 2014). Also known as B2M, it has been suggested that this protein can be used as a prognostic marker for HIV infection (Halota et al. 2002).

Mouse anti Human beta 2 microglobulin antibody recognizes β2 microglobulin as a band of ~12 kDa in HeLa cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti beta 2 microglobulin antibody detects a band of approximately 12 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
5% Glycerol
Immunogen
Highly pure human beta 2 microglobulin from urine

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P61769
Entrez Gene
B2M
GO Terms
GO:0000139 Golgi membrane
GO:0005515 protein binding
GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
GO:0042612 MHC class I protein complex
GO:0006955 immune response
GO:0016032 viral reproduction
GO:0031901 early endosome membrane
GO:0050690 regulation of defense response to virus by virus
GO:0060333 interferon-gamma-mediated signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Beta 2 Microglobulin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
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