Bcl-6 antibody | OTI20A3

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Mouse anti Bcl-6

BCL6 antibody recognizes B-cell lymphoma 6 protein, also known as B-cell lymphoma 5 protein, B-cell lymphoma 6 protein, B-cell lymphoma 6 protein transcript, BCL-5, BCL-6, cys-his2 zinc finger transcription factor, or lymphoma-associated zinc finger gene.

Bcl-6 Antibody with Control Lysate

BCL6 antibody recognizes B-cell lymphoma 6 protein, also known as B-cell lymphoma 5 protein, B-cell lymphoma 6 protein, B-cell lymphoma 6 protein transcript, BCL-5, BCL-6, cys-his2 zinc finger transcription factor, or lymphoma-associated zinc finger gene.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI20A3
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00325 WB 100 µl
VMA00325KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human Bcl-6 antibody recognizes Bcl-6, also known as B-cell lymphoma 6 protein, B-cell lymphoma 5 protein, B-cell lymphoma 6 protein transcript, cys-his2 zinc finger transcription factor, lymphoma-associated zinc finger gene on chromosome 3, protein LAZ-3, zinc finger and BTB domain-containing protein 27, zinc finger protein 51 and zinc finger transcription factor BCL6S.

Encoded by BCL6 gene, the B-cell lymphoma 6 protein is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. BCL6 is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for BCL6 (provided by RefSeq, Sep 2008).

Mouse anti Human Bcl-6 protein antibody detects a band of 98 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti Bcl-6 detects a band of approximately 98 kDa in Raji cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-300 of human Bcl-6 (NP_001697) produced in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Raji lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P41182 Related reagents
Entrez Gene
BCL6 Related reagents
GO Terms
GO:0000060 protein import into nucleus, translocation
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0005515 protein binding
GO:0002634 regulation of germinal center formation
GO:0002903 negative regulation of B cell apoptosis
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005634 nucleus
GO:0006974 response to DNA damage stimulus
GO:0008270 zinc ion binding
GO:0016564 transcription repressor activity
GO:0030308 negative regulation of cell growth
GO:0043065 positive regulation of apoptosis
GO:0043565 sequence-specific DNA binding
GO:0045749 negative regulation of S phase of mitotic cell cycle
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Bcl-6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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