Bcl-2 antibody

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Mouse anti Bcl-2

Bcl-2 antibody recognizes Apoptosis regulator bcl-2. The antibody has been extensively validated for western blotting using whole cell lysates.

Bcl-2 Antibody with Control Lysate

Bcl-2 antibody recognizes Apoptosis regulator bcl-2. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00017 WB 100 µl
VMA00017KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human bcl-2 antibody recognizes the human apoptosis regulator bcl-2 oncoprotein, a 239 amino acid integral single pass membrane protein containing 4 BH motifs which lies within the cell rather than on the cell surface. The protein is localized to the outer mitochondrial membrane and plays a role in the inhibition of apoptosis.

Mouse anti Human bcl-2 antibody reacts with small B lymphocytes in the mantle zone and many cells within T cell areas. Very few cells in germinal centres are stained. In the thymus many cells in the medulla are stained but the cortex shows weak or negative staining. In non-hematopoietic tissues few cells are stained (Krajewski et al. 1995). The antibody reacts with neoplastic cells of follicular lymphoma and anaplastic large cell lymphoma (Reed 1997).

Mouse anti Human bcl-2 antibody recognizes bcl-2 as a ~26 kDa single band by western blotting in various human cell lysates and the COS-7 cell line.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
African green monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 41-54 of human bcl-2

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P10415 Related reagents
Entrez Gene
BCL2 Related reagents
GO Terms
GO:0000209 protein polyubiquitination
GO:0001836 release of cytochrome c from mitochondria
GO:0002020 protease binding
GO:0016021 integral to membrane
GO:0005741 mitochondrial outer membrane
GO:0005789 endoplasmic reticulum membrane
GO:0006916 anti-apoptosis
GO:0006959 humoral immune response
GO:0006974 response to DNA damage stimulus
GO:0007565 female pregnancy
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0042493 response to drug
GO:0009636 response to toxin
GO:0010039 response to iron ion
GO:0016563 transcription activator activity
GO:0030307 positive regulation of cell growth
GO:0030890 positive regulation of B cell proliferation
GO:0031965 nuclear membrane
GO:0032848 negative regulation of cellular pH reduction
GO:0034097 response to cytokine stimulus
GO:0035094 response to nicotine
GO:0042100 B cell proliferation
GO:0042803 protein homodimerization activity
GO:0043496 regulation of protein homodimerization activity
GO:0043497 regulation of protein heterodimerization activity
GO:0043524 negative regulation of neuron apoptosis
GO:0043565 sequence-specific DNA binding
GO:0046902 regulation of mitochondrial membrane permeability
GO:0046982 protein heterodimerization activity
GO:0050853 B cell receptor signaling pathway
GO:0051402 neuron apoptosis
GO:0051434 BH3 domain binding
GO:0051607 defense response to virus
GO:0051881 regulation of mitochondrial membrane potential
GO:0051902 negative regulation of mitochondrial depolarization
GO:0070059 apoptosis in response to endoplasmic reticulum stress
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Bcl-2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti bcl-2 antibody detects a band of approximately 26 kDa in Jurkat cell lysates.
Western Blotting
Anti bcl-2 antibody detects a band of approximately 26 kDa in Jurkat cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB