Bcl-2 antibody | 100

100% Secure

Mouse anti Bcl-2

Product Type
PrecisionAb Monoclonal
Product Code Applications Pack Size List Price Quantity
100 µl loader

Mouse anti Human bcl-2 antibody recognizes the human apoptosis regulator bcl-2 oncoprotein, a 239 amino acid integral single pass membrane protein containing 4 BH motifs which lies within the cell rather than on the cell surface. The protein is localized to the outer mitochondrial membrane and plays a role in the inhibition of apoptosis.

Mouse anti Human bcl-2 antibody reacts with small B lymphocytes in the mantle zone and many cells within T cell areas. Very few cells in germinal centres are stained. In the thymus many cells in the medulla are stained but the cortex shows weak or negative staining. In non-hematopoietic tissues few cells are stained (Krajewski et al. 1995). The antibody reacts with neoplastic cells of follicular lymphoma and anaplastic large cell lymphoma (Reed 1997).

Mouse anti Human bcl-2 antibody recognizes bcl-2 as a ~26 kDa single band by western blotting in various human cell lysates and the COS-7 cell line.

Product Details

Target Species
Western Blotting
Anti bcl-2 antibody detects a band of approximately 26 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
African green monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Synthetic peptide corresponding to amino acids 41-54 of human bcl-2
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0000209 protein polyubiquitination
GO:0001836 release of cytochrome c from mitochondria
GO:0002020 protease binding
GO:0016021 integral to membrane
GO:0005741 mitochondrial outer membrane
GO:0005789 endoplasmic reticulum membrane
GO:0006916 anti-apoptosis
GO:0006959 humoral immune response
GO:0006974 response to DNA damage stimulus
GO:0007565 female pregnancy
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0042493 response to drug
GO:0009636 response to toxin
GO:0010039 response to iron ion
GO:0016563 transcription activator activity
GO:0030307 positive regulation of cell growth
GO:0030890 positive regulation of B cell proliferation
GO:0031965 nuclear membrane
GO:0032848 negative regulation of cellular pH reduction
GO:0034097 response to cytokine stimulus
GO:0035094 response to nicotine
GO:0042100 B cell proliferation
GO:0042803 protein homodimerization activity
GO:0043496 regulation of protein homodimerization activity
GO:0043497 regulation of protein heterodimerization activity
GO:0043524 negative regulation of neuron apoptosis
GO:0043565 sequence-specific DNA binding
GO:0046902 regulation of mitochondrial membrane permeability
GO:0046982 protein heterodimerization activity
GO:0050853 B cell receptor signaling pathway
GO:0051402 neuron apoptosis
GO:0051434 BH3 domain binding
GO:0051607 defense response to virus
GO:0051881 regulation of mitochondrial membrane potential
GO:0051902 negative regulation of mitochondrial depolarization
GO:0070059 apoptosis in response to endoplasmic reticulum stress
PrecisionAb is a trademark of Bio-Rad Laboratories.
For research purposes only

Applications of Bcl-2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer?

Watch the Tool Tutorial Video ▸

  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra