Bcl-10 antibody | 151

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Mouse anti Human Bcl-10:Alexa Fluor®488

Mouse anti Human Bcl-10

Mouse anti Human Bcl-10:RPE

Product Type
Monoclonal Antibody
Clone
151
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2750A488 F* 100 Tests/1ml
MCA2750GA E F* IP P* WB 0.1 mg
MCA2750PE F* 100 Tests
Mouse anti Human Bcl-10 antibody, clone 151 recognizes human B-cell lymphoma/leukaemia 10 (Bcl-10), a 31kD CARD (caspase recruitment domain) containing molecule that is involved in the regulation of apoptosis. Bcl-10 was originally identified through its direct involvement in t(1;14)(p22;q32) of mucosa-associated lymphoid tissue (MALT) lymphoma. The Bcl-10 molecule is predominantly located in the cytoplasm but expression has also been reported in the nucleus of some lymphomas.

Reports suggest that Bcl-10 is essential for nuclear factor (NF)-kappaB activation after lymphocyte receptor antigen stimulation and for the development of mature B-cells via ubiquitination of NEMO (Zhouet al. 2004).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor 488 - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
Full length recombinant Bcl-10.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
O95999 Related reagents
Entrez Gene
BCL10 Related reagents
GO Terms
GO:0001843 neural tube closure
GO:0002224 toll-like receptor signaling pathway
GO:0002237 response to molecule of bacterial origin
GO:0002906 negative regulation of mature B cell apoptosis
GO:0003713 transcription coactivator activity
GO:0006915 apoptosis
GO:0005764 lysosome
GO:0005829 cytosol
GO:0005634 nucleus
GO:0008022 protein C-terminus binding
GO:0031398 positive regulation of protein ubiquitination
GO:0031625 ubiquitin protein ligase binding
GO:0032449 CBM complex
GO:0032765 positive regulation of mast cell cytokine production
GO:0042109 lymphotoxin A biosynthetic process
GO:0042226 interleukin-6 biosynthetic process
GO:0042327 positive regulation of phosphorylation
GO:0051260 protein homooligomerization
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043130 ubiquitin binding
GO:0043422 protein kinase B binding
GO:0043621 protein self-association
GO:0045087 innate immune response
GO:0045121 membrane raft
GO:0045416 positive regulation of interleukin-8 biosynthetic process
GO:0045893 positive regulation of transcription, DNA-dependent
GO:0048471 perinuclear region of cytoplasm
GO:0050852 T cell receptor signaling pathway
GO:0051059 NF-kappaB binding
GO:0051092 positive regulation of NF-kappaB transcription factor activity
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of Bcl-10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat 1/10
ELISA
Flow Cytometry 1
Immunohistology - Paraffin 2 1/50 1/800
Immunoprecipitation
Western Blotting
Flow Cytometry 1 Neat
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucopermâ„¢ (Product Code BUF09) for this purpose.
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucopermâ„¢ (Product Code BUF09) for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucopermâ„¢ (Product Code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Human tonsil
Western Blotting
MCA2750GA detects bands of approximately 32kDa and 37kDa in cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 488 MCA928A488 100 Tests/1ml F
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Histar Detection System STAR3000B 150 Tests C P
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for Bcl-10 antibody

  1. Ye, H. et al. (2005) MALT lymphoma with t(14;18)(q32;q21)/IGH-MALT1 is characterized by strong cytoplasmic MALT1 and BCL10 expression.
    J Pathol. 205:293 – 301.
  2. Ye, H. et al. (2000) BCL10 expression in normal and neoplastic lymphoid tissue. Nuclear localization in MALT lymphoma.
    Am J Pathol. 157:1147-54.