BAX antibody | 2D2

100% Secure

Mouse anti Human BAX (N-Terminal)

Product Type
Monoclonal Antibody
Clone
2D2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2738 E IF IP WB 0.1 mg
Mouse anti Human BAX antibody, clone 2D2 recognizes an epitope within the N-terminal (NT) region of human apoptosis regulator BAX, also known as Bcl-2-like protein 4. BAX is a pro-apoptotic member of the Bcl-2 family. BAX accelerates programmed cell death by binding to and antagonizing the apoptosis repressor Bcl-2< or its adenovirus homolog E1B 19k protein. It also induces the release of cytochrome C , activation of caspase-3 and thereby apoptosis. Defects in BAX are found in some cell lines from hematopoietic malignancies as T-cell acute lymphoblastic leukemia, Burkitt lymphoma, and plasmacytoma (Brimmell et al. 1998).

Mouse anti Human BAX antibody, clone 2D2 has been successfully used for the immunoprecipitation of bovine BAX from thymus (Basañez et al. 1999).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
African green monkey
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
A synthetic peptide corresponding to the N-terminal region (aa 3-16) of human Bax having the sequence C-GSGEQPRGGGPTSS.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Splenocytes from mice immunized with KLH-conjugated BAX peptide were fused with cells of the murine NS-1 myeloma.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q07812 Related reagents
Entrez Gene
BAX Related reagents
Regulatory
For research purposes only

Applications of BAX antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 1.0ug/ml
Immunofluorescence 0.5 1.0ug/ml
Immunoprecipitation
Western Blotting 0.5 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2738 detects a band of approximately 23kDa in COLO320 DM cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Functional Assays

Immunoprecipitation

Western Blotting

Product Specific References

References for BAX antibody

  1. Hsu, Y.T. and Youle, R.J. (1997) Nonionic detergents induce dimerization among members of the Bcl-2 family.
    J Biol Chem. 272: 13829-34.
  2. Hsu, Y.T. et al. (1997) Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis.
    Proc Natl Acad Sci U S A. 94 (8): 3668-72.
  3. Wolter, K.G. et al. (1997) Movement of Bax from the cytosol to mitochondria during apoptosis.
    J Cell Biol.139: 1281-92.
  4. Zhang, Y. et al. (2002) Selective cytotoxicity of intracellular amyloid beta peptide1-42 through p53 and Bax in cultured primary human neurons.
    J Cell Biol. 156: 519-29.
  5. Basañez, G. et al. (1999) Bax, but not Bcl-xL, decreases the lifetime of planar phospholipid bilayer membranes at subnanomolar concentrations.
    Proc Natl Acad Sci U S A. 96: 5492-7.
  6. Yethon, J.A. et al. (2003) Interaction with a membrane surface triggers a reversible conformational change in Bax normally associated with induction of apoptosis.
    J Biol Chem. 278: 48935-41.
  7. Liu, F.T. et al. (2003) Bax conformational change is a crucial step for PUMA-mediated apoptosis in human leukemia.
    Biochem Biophys Res Commun. 310: 956-62.
  8. Edlich F et al. (2011) Bcl-x(L) retrotranslocates Bax from the mitochondria into the cytosol.
    Cell. 145: 104-16.
  9. Zhou, H. et al. (2007) Complete activation of Bax by a single site mutation.
    Oncogene. 26: 7092-102.
Mitochondrial dynamics logo

New Mitochondrial Dynamics Poster

Learn about the mitochondrial fusion-division cycle and how aberrant regulation results in disease

Download or order your free poster