BAX antibody | 1F5-1B7
Mouse anti Human BAX antibody, clone 1F5-1B7 recognizes human Apoptosis regulator BAX, also known as BAX or Apoptosis regulator BAX, Bax is a 192 amino acid ~21 kDa proapoptotic member of the Bcl-2 family. BAX accelerates programmed cell death by binding to and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. It also induces the release of cytochrome C , activation of CASP3 and thereby apoptosis. Defects in BAX are found in some cell lines from hematopoietic malignancies as T-cell acute lymphoblastic leukemia, Burkitt lymphoma, and plasmacytoma.
Multiple potential isoforms of BAX generated by alternative splicing have been described. Mouse anti Human BAX antibody, clone 1F5-1B7 binds to the canonical α isoform, however binding to other potential isoforms β, γ, δ, ε, ζ, ψ, and σ, all of which demonstrate variation within the immunogen sequence, has not been evaluated.
Multiple potential isoforms of BAX generated by alternative splicing have been described. Mouse anti Human BAX antibody, clone 1F5-1B7 binds to the canonical α isoform, however binding to other potential isoforms β, γ, δ, ε, ζ, ψ, and σ, all of which demonstrate variation within the immunogen sequence, has not been evaluated.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein with a GST tag corresponding to amino acids 1 - 193 of human BAX.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
Applications of BAX antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 1 | 10 ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
References for BAX antibody
-
Wnęk A et al. (2017) Molecular and immunohistochemical expression of apoptotic proteins Bax, Bcl-2 and Caspase 3 in infantile hemangioma tissues as an effect of propranolol treatment.
Immunol Lett. pii: S0165-2478(16)30248-6. [Epub ahead of print]
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