BAX antibody | 1F5-1B7
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|Mouse anti Human BAX antibody, clone 1F5-1B7 recognizes human Apoptosis regulator BAX, also known as BAX or Apoptosis regulator BAX, Bax is a 192 amino acid ~21 kDa proapoptotic member of the Bcl-2 family. BAX accelerates programmed cell death by binding to and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. It also induces the release of cytochrome C , activation of CASP3 and thereby apoptosis. Defects in BAX are found in some cell lines from hematopoietic malignancies as T-cell acute lymphoblastic leukemia, Burkitt lymphoma, and plasmacytoma.
Multiple potential isoforms of BAX generated by alternative splicing have been described. Mouse anti Human BAX antibody, clone 1F5-1B7 binds to the canonical α isoform, however binding to other potential isoforms β, γ, δ, ε, ζ, ψ, and σ, all of which demonstrate variation within the immunogen sequence, has not been evaluated.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Recombinant protein with a GST tag corresponding to amino acids 1 - 193 of human BAX.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1||1||10 ug/ml|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
References for BAX antibody
Wnęk A et al. (2017) Molecular and immunohistochemical expression of apoptotic proteins Bax, Bcl-2 and Caspase 3 in infantile hemangioma tissues as an effect of propranolol treatment.
Immunol Lett. pii: S0165-2478(16)30248-6. [Epub ahead of print]
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