ATP6V1E1 antibody | AB03/4H1
Filter by Application:
WB ResetMouse anti ATP6V1E1
- Product Type
- PrecisionAb Monoclonal
- Clone
- AB03/4H1
- Isotype
- IgG1
- Format
- Purified
- Specificity
- ATP6V1E1
Antibody Quality and Performance Guarantee
| Mouse anti ATP6V1E1 antibody recognizes V-type proton ATPase subunit E 1. ATP6V1E1 encodes the E1 subunit of the V-ATPase, a proton pump which couples ATP hydrolysis to active proton transport across membranes (Van Damme et al. 2017). The V-ATPase can acidify lysosomes/vacuoles, Golgi, and endosomes, and also functions in proton export from the cell. The complex is also important for multiple cellular processes including protein processing, endocytosis and autophagy (Hayek et al. 2019). Mutations in the gene encoding ATP6V1E1 cause autosomal-recessive Cutis Laxa, a condition characterized by redundant or wrinkly skin and elastic-fiber fragmentation (Van Damme et al. 2017). |
|
- Target Species
- Human
- Western Blotting
- Mouse anti ATP6V1E1 detects a band of approximately 29 kDa in HepG2 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-226 of human ATP6V1E1
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
 |
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- P36543
- Entrez Gene
- ATP6V1E1
- GO Terms
- GO:0005515 protein binding
- GO:0046961 proton-transporting ATPase activity, rotational mechanism
- GO:0005829 cytosol
- GO:0005768 endosome
- GO:0006879 cellular iron ion homeostasis
- GO:0008286 insulin receptor signaling pathway
- GO:0015986 ATP synthesis coupled proton transport
- GO:0016324 apical plasma membrane
- GO:0033178 proton-transporting two-sector ATPase complex, catalytic domain
- View More GO Terms
- GO:0033572 transferrin transport
VMA00944
100005721
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Request a different product with this specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up