ATP6V1B2 antibody | OTI1E11

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Mouse anti ATP6V1B2

Product Type
PrecisionAb Monoclonal
Product Code Applications Pack Size List Price Quantity
100 µl loader

Mouse anti Human ATP6V1B2 antibody, clone OTI1E11 recognizes ATP6V1B2, brain isoform, also known as H+ transporting two-sector ATPase, V-ATPase B2 subunit, V-ATPase subunit B 2, V-type proton ATPase subunit B, brain isoform, endomembrane proton pump 58 kDa subunit, vacuolar H+-ATPase 56,000 subunit and vacuolar proton pump subunit B 2.

ATP6V1B2 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by ATP6V1B2 is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts (provided by RefSeq, Jul 2008).

Mouse anti Human ATP6V1B2 antibody, clone OTI1E11 detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Western Blotting
Anti ATP6V1B2 detects a band of approximately 52 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Full length recombinant human ATP6V1B2 (NP_001684) produced in HEK293T cells

Storage Information

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0005886 plasma membrane
GO:0046961 proton-transporting ATPase activity, rotational mechanism
GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism
GO:0005829 cytosol
GO:0005794 Golgi apparatus
GO:0006879 cellular iron ion homeostasis
GO:0008286 insulin receptor signaling pathway
GO:0012505 endomembrane system
GO:0015986 ATP synthesis coupled proton transport
GO:0033180 proton-transporting V-type ATPase, V1 domain
GO:0033572 transferrin transport
GO:0042470 melanosome
PrecisionAb is a trademark of Bio-Rad Laboratories.
For research purposes only

Applications of ATP6V1B2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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