ATP6V1A antibody | AB01/4F4

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Mouse anti ATP6V1A

Product Type
PrecisionAb Monoclonal
Clone
AB01/4F4
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00933
Datasheet
IP WB
SDS
100 µl loader

Mouse anti ATP6V1A antibody recognizes V-type proton ATPase catalytic subunit A, also known as ATP6A1, ATP6V1A1, and VPP2.

ATP6V1A forms the A subunit of the V-ATPase complex, essential for maintaining the correct pH of endosomes, lysosomes, and other intracellular compartments (Smith et al. 2003). The gene encoding ATP6V1A is widely-expressed, and inherited mutations cause cutis laxa symptoms affecting the central nervous system and conferring a risk of life-threatening cardiopulmonary complications (Damme et al. 2017). Expression of ATP6V1A is regulated by the transcription factor YY1, which has a variety of functions and is upregulated in cervical carcinoma. Elevated expression of ATP6V1A is observed in gastric cancer, but gastric cancer patients with elevated ATP6V1A also have a better prognosis (Wang et al. 2017).

Product Details

Target Species
Human
Western Blotting
Mouse anti ATP6V1A detects a band of approximately 72 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-350 of human ATP6V1A
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P38606
Entrez Gene
ATP6V1A
GO Terms
GO:0046961 proton-transporting ATPase activity, rotational mechanism
GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism
GO:0005829 cytosol
GO:0005887 integral to plasma membrane
GO:0005524 ATP binding
GO:0006879 cellular iron ion homeostasis
GO:0008286 insulin receptor signaling pathway
GO:0015986 ATP synthesis coupled proton transport
GO:0033180 proton-transporting V-type ATPase, V1 domain
GO:0033572 transferrin transport
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of ATP6V1A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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