ATP6V1A antibody | AB01/4F4
siRNA Validated
IP-MS Validated
Mouse anti ATP6V1A
- Product Type
- PrecisionAb Monoclonal
- Clone
- AB01/4F4
- Isotype
- IgG1
- Format
- Purified
- Specificity
- ATP6V1A
Antibody Quality and Performance Guarantee
| Mouse anti ATP6V1A antibody recognizes V-type proton ATPase catalytic subunit A, also known as ATP6A1, ATP6V1A1, and VPP2. ATP6V1A forms the A subunit of the V-ATPase complex, essential for maintaining the correct pH of endosomes, lysosomes, and other intracellular compartments (Smith et al. 2003). The gene encoding ATP6V1A is widely-expressed, and inherited mutations cause cutis laxa symptoms affecting the central nervous system and conferring a risk of life-threatening cardiopulmonary complications (Damme et al. 2017). Expression of ATP6V1A is regulated by the transcription factor YY1, which has a variety of functions and is upregulated in cervical carcinoma. Elevated expression of ATP6V1A is observed in gastric cancer, but gastric cancer patients with elevated ATP6V1A also have a better prognosis (Wang et al. 2017). |
|
- Target Species
- Human
- Western Blotting
- Mouse anti ATP6V1A detects a band of approximately 72 kDa in HeLa cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-350 of human ATP6V1A
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation |  |
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| Western Blotting | |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- P38606
- Entrez Gene
- ATP6V1A
- GO Terms
- GO:0046961 proton-transporting ATPase activity, rotational mechanism
- GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism
- GO:0005829 cytosol
- GO:0005887 integral to plasma membrane
- GO:0005524 ATP binding
- GO:0006879 cellular iron ion homeostasis
- GO:0008286 insulin receptor signaling pathway
- GO:0015986 ATP synthesis coupled proton transport
- GO:0033180 proton-transporting V-type ATPase, V1 domain
- View More GO Terms
- GO:0033572 transferrin transport
VMA00933
100005648
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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