ATP5C1 antibody | OTI2H6
Mouse anti Human ATP5C1 antibody, clone OTI2H6 recognizes the ATP5C1, also known as ATP synthase gamma chain, mitochondrial, ATP synthase subunit gamma, mitochondrial, F-ATPase gamma subunit, mitochondrial ATP synthase and gamma subunit 1.
Encoded by the ATP5C1 gene, this protein a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5C1 encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. ATP5C1 also has a pseudogene on chromosome 14 (provided by RefSeq, Jul 2008).
Mouse anti Human ATP5C1 antibody, clone OTI2H6 detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the ATP5C1 gene, this protein a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5C1 encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. ATP5C1 also has a pseudogene on chromosome 14 (provided by RefSeq, Jul 2008).
Mouse anti Human ATP5C1 antibody, clone OTI2H6 detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti ATP5C1 detects a band of approximately 30 kDa in HeLa cell lysates.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human ATP5C1 (NP_001001973) produced in E. coli
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P36542
- Entrez Gene
- ATP5C1
- GO Terms
- GO:0046961 proton-transporting ATPase activity, rotational mechanism
- GO:0000275 mitochondrial proton-transporting ATP synthase complex, catalytic core F(1)
- GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism
- GO:0005759 mitochondrial matrix
- GO:0022904 respiratory electron transport chain
- GO:0006119 oxidative phosphorylation
- GO:0042776 mitochondrial ATP synthesis coupled proton transport
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of ATP5C1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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