ATP5C1 antibody | OTI2H6

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Mouse anti ATP5C1

ATP5C1 antibody recognizes the gamma subunit of mitochondrial ATP synthase, also known as F-ATPase gamma subunit. This antibody has been extensively validated for western blotting using whole cell lysates.

ATP5C1 Antibody with Control Lysate

ATP5C1 antibody recognizes the gamma subunit of mitochondrial ATP synthase, also known as F-ATPase gamma subunit. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI2H6
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00188 WB 100 µl
VMA00188KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human ATP5C1 antibody recognizes the ATP5C1, also known as ATP synthase gamma chain, mitochondrial, ATP synthase subunit gamma, mitochondrial, F-ATPase gamma subunit, mitochondrial ATP synthase and gamma subunit 1.

Encoded by the ATP5C1 gene, this protein a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5C1 encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. ATP5C1 also has a pseudogene on chromosome 14 (provided by RefSeq, Jul 2008).

Mouse anti Human ATP5C1 antibody detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human ATP5C1 (NP_001001973) produced in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P36542 Related reagents
Entrez Gene
ATP5C1 Related reagents
GO Terms
GO:0046961 proton-transporting ATPase activity, rotational mechanism
GO:0000275 mitochondrial proton-transporting ATP synthase complex, catalytic core F(1)
GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism
GO:0005759 mitochondrial matrix
GO:0022904 respiratory electron transport chain
GO:0006119 oxidative phosphorylation
GO:0042776 mitochondrial ATP synthesis coupled proton transport
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ATP5C1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti ATP5C1 detects a band of approximately 30 kDa in HeLa cell lysates.
Western Blotting
Anti ATP5C1 detects a band of approximately 30 kDa in HeLa cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB