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ATP5C1 antibody | OTI2H6

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Anti ATP5C1 Antibody, clone OTI2H6 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti ATP5C1 Antibody, clone OTI2H6 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti ATP5C1 Antibody, clone OTI2H6 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Mouse anti Human ATP5C1 antibody, clone OTI2H6 (VMA00188) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 17 second exposure. Arrow points to ATP5C1 (molecular weight 30 kDa).
Anti ATP5C1 Antibody, clone OTI2H6 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of ATP5C1 from HeLa whole cell lysate. The Input lane is 10% input; the ATP5C1-IP lane is the immunoprecipitation with the ATP5C1 antibody (2.5 µg, VMA00188); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using ATP5C1 antibody (1/1,000, VMA00188) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to ATP5C1.

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WB IP

Mouse anti ATP5C1

Product Type
PrecisionAb Monoclonal
Clone
OTI2H6
Isotype
IgG1
Format
Purified
Specificity
ATP5C1

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Product Code Applications Pack Size List Price Your Price Qty
VMA00188
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IP WB 100 µl loader
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Mouse anti Human ATP5C1 antibody, clone OTI2H6 recognizes the ATP5C1, also known as ATP synthase gamma chain, mitochondrial, ATP synthase subunit gamma, mitochondrial, F-ATPase gamma subunit, mitochondrial ATP synthase and gamma subunit 1.

Encoded by the ATP5C1 gene, this protein a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5C1 encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. ATP5C1 also has a pseudogene on chromosome 14 (provided by RefSeq, Jul 2008).

Mouse anti Human ATP5C1 antibody, clone OTI2H6 detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti ATP5C1 detects a band of approximately 30 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human ATP5C1 (NP_001001973) produced in E. coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P36542
Entrez Gene
ATP5C1
GO Terms
GO:0046961 proton-transporting ATPase activity, rotational mechanism
GO:0000275 mitochondrial proton-transporting ATP synthase complex, catalytic core F(1)
GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism
GO:0005759 mitochondrial matrix
GO:0022904 respiratory electron transport chain
GO:0006119 oxidative phosphorylation
GO:0042776 mitochondrial ATP synthesis coupled proton transport
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