ATP5B antibody | OTI1F7

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Anti ATP5B Antibody, clone OTI1F7 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti ATP5B Antibody, clone OTI1F7 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti ATP5B Antibody, clone OTI1F7 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti ATP5B Antibody, clone OTI1F7 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human ATP5B antibody, clone OTI1F7 (**VMA00114**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 60 second exposure. Arrow points to ATP5B (molecular weight 57 kDa).

Anti ATP5B Antibody, clone OTI1F7 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against ATP5B (1 & 2) or a scrambled siRNA (-) probed with (A) Mouse anti ATP5B antibody (**VMA00114**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 1705047) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 66 second and (B) 30 second exposure.

Anti ATP5B Antibody, clone OTI1F7 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of ATP5B from whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against ATP5B (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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Mouse anti ATP5B

Product Type: PrecisionAb Monoclonal
Clone: OTI1F7
Isotype: IgG1
Format: Purified
Specificity: ATP5B

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VMA00114 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human ATP5B antibody, clone OTI1F7 recognizes ATP5B, also known as ATP synthase subunit beta, mitochondrial, epididymis secretory protein Li 271, mitochondrial ATP synthase beta subunit and mitochondrial ATP synthetase, beta subunit.

Encoded by the ATP5B gene, ATP synthase subunit beta is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5B encodes the beta subunit of the catalytic core (provided by RefSeq, Jul 2008).

Mouse anti Human ATP5B antibody detects a band of 57 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti ATP5B detects a band of approximately 57 kDa in HeLa cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human ATP5B (NP_001677) produced in HEK293T cells
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P06576
Entrez Gene: ATP5B
GO Terms: GO:0005886 plasma membrane; GO:0046961 proton-transporting ATPase activity, rotational mechanism; GO:0001525 angiogenesis; GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism; GO:0005524 ATP binding; GO:0005754 mitochondrial proton-transporting ATP synthase, catalytic core; GO:0022904 respiratory electron transport chain; GO:0008553 hydrogen-exporting ATPase activity, phosphorylative mechanism; GO:0009986 cell surface; View More GO Terms; GO:0042288 MHC class I protein binding; GO:0042645 mitochondrial nucleoid; GO:0042776 mitochondrial ATP synthesis coupled proton transport; GO:0043499 eukaryotic cell surface binding; GO:0051453 regulation of intracellular pH