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ATP5B antibody | OTI1F7

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siRNA Validated
Anti ATP5B Antibody, clone OTI1F7 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti ATP5B Antibody, clone OTI1F7 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti ATP5B Antibody, clone OTI1F7 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti ATP5B Antibody, clone OTI1F7 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Mouse anti Human ATP5B antibody, clone OTI1F7 (VMA00114) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 60 second exposure. Arrow points to ATP5B (molecular weight 57 kDa).
Anti ATP5B Antibody, clone OTI1F7 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against ATP5B (1 & 2) or a scrambled siRNA (-) probed with (A) Mouse anti ATP5B antibody (VMA00114) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 1705047) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 66 second and (B) 30 second exposure.
Anti ATP5B Antibody, clone OTI1F7 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of ATP5B from whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against ATP5B (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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WB

Mouse anti ATP5B

Product Type
PrecisionAb Monoclonal
Clone
OTI1F7
Isotype
IgG1
Format
Purified
Specificity
ATP5B

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Product Code Applications Pack Size List Price Your Price Qty
VMA00114
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
List Price Your Price
loader

Mouse anti Human ATP5B antibody, clone OTI1F7 recognizes ATP5B, also known as ATP synthase subunit beta, mitochondrial, epididymis secretory protein Li 271, mitochondrial ATP synthase beta subunit and mitochondrial ATP synthetase, beta subunit.

Encoded by the ATP5B gene, ATP synthase subunit beta is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5B encodes the beta subunit of the catalytic core (provided by RefSeq, Jul 2008).

Mouse anti Human ATP5B antibody detects a band of 57 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti ATP5B detects a band of approximately 57 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human ATP5B (NP_001677) produced in HEK293T cells
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P06576
Entrez Gene
ATP5B
GO Terms
GO:0005886 plasma membrane
GO:0046961 proton-transporting ATPase activity, rotational mechanism
GO:0001525 angiogenesis
GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism
GO:0005524 ATP binding
GO:0005754 mitochondrial proton-transporting ATP synthase, catalytic core
GO:0022904 respiratory electron transport chain
GO:0008553 hydrogen-exporting ATPase activity, phosphorylative mechanism
GO:0009986 cell surface
GO:0042288 MHC class I protein binding
GO:0042645 mitochondrial nucleoid
GO:0042776 mitochondrial ATP synthesis coupled proton transport
GO:0043499 eukaryotic cell surface binding
GO:0051453 regulation of intracellular pH

VMA00114

240615

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