ATP5B antibody | OTI1F7

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Anti ATP5B Antibody, clone OTI1F7 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with ATP5B antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 60 second exposure. Arrow points to ATP5B (molecular weight 57 kDa)

Mouse anti ATP5B

ATP5B antibody recognizes mitochondrial ATP synthase subunit beta, also known as mitochondrial epididymis secretory protein Li 271 or mitochondrial ATP synthetase. This antibody has been extensively validated for western blotting using whole cell lysates.

ATP5B Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: OTI1F7
Isotype: IgG1

Product Code	Applications	Pack Size
VMA00114	WB	100 µl
VMA00114KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human ATP5B antibody recognizes ATP5B, also known as ATP synthase subunit beta, mitochondrial, epididymis secretory protein Li 271, mitochondrial ATP synthase beta subunit and mitochondrial ATP synthetase, beta subunit.

Encoded by the ATP5B gene, ATP synthase subunit beta is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5B encodes the beta subunit of the catalytic core (provided by RefSeq, Jul 2008).

Mouse anti Human ATP5B antibody detects a band of 57 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography from ascites.

Preparation

20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Immunogen

Full length recombinant human ATP5B (NP_001677) produced in HEK293T cells

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P06576 Related reagents
Entrez Gene: ATP5B Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0046961 proton-transporting ATPase activity, rotational mechanism; GO:0001525 angiogenesis; GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism; GO:0005524 ATP binding; GO:0005754 mitochondrial proton-transporting ATP synthase, catalytic core; GO:0022904 respiratory electron transport chain; GO:0008553 hydrogen-exporting ATPase activity, phosphorylative mechanism; GO:0009986 cell surface; GO:0042288 MHC class I protein binding; GO:0042645 mitochondrial nucleoid; GO:0042776 mitochondrial ATP synthesis coupled proton transport; GO:0043499 eukaryotic cell surface binding; GO:0051453 regulation of intracellular pH
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of ATP5B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Western Blotting: Anti ATP5B detects a band of approximately 57 kDa in HeLa cell lysates.
Western Blotting: Anti ATP5B detects a band of approximately 57 kDa in HeLa cell lysates.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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