ATP5B antibody | OTI1F7
Mouse anti ATP5B
ATP5B antibody recognizes mitochondrial ATP synthase subunit beta, also known as mitochondrial epididymis secretory protein Li 271 or mitochondrial ATP synthetase. This antibody has been extensively validated for western blotting using whole cell lysates.
ATP5B Antibody with Control Lysate
ATP5B antibody recognizes mitochondrial ATP synthase subunit beta, also known as mitochondrial epididymis secretory protein Li 271 or mitochondrial ATP synthetase. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI1F7
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00114 | WB | 100 µl | ||||
| VMA00114KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human ATP5B antibody recognizes ATP5B, also known as ATP synthase subunit beta, mitochondrial, epididymis secretory protein Li 271, mitochondrial ATP synthase beta subunit and mitochondrial ATP synthetase, beta subunit.
Encoded by the ATP5B gene, ATP synthase subunit beta is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5B encodes the beta subunit of the catalytic core (provided by RefSeq, Jul 2008).
Mouse anti Human ATP5B antibody detects a band of 57 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the ATP5B gene, ATP synthase subunit beta is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5B encodes the beta subunit of the catalytic core (provided by RefSeq, Jul 2008).
Mouse anti Human ATP5B antibody detects a band of 57 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti ATP5B detects a band of approximately 57 kDa in HeLa cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human ATP5B (NP_001677) produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P06576 Related reagents
- Entrez Gene
- ATP5B Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0046961 proton-transporting ATPase activity, rotational mechanism
- GO:0001525 angiogenesis
- GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism
- GO:0005524 ATP binding
- GO:0005754 mitochondrial proton-transporting ATP synthase, catalytic core
- GO:0022904 respiratory electron transport chain
- GO:0008553 hydrogen-exporting ATPase activity, phosphorylative mechanism
- GO:0009986 cell surface
- GO:0042288 MHC class I protein binding
- GO:0042645 mitochondrial nucleoid
- GO:0042776 mitochondrial ATP synthesis coupled proton transport
- GO:0043499 eukaryotic cell surface binding
- GO:0051453 regulation of intracellular pH
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of ATP5B antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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