ATP5B antibody | OTI1F7

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Mouse anti ATP5B

ATP5B antibody recognizes mitochondrial ATP synthase subunit beta, also known as mitochondrial epididymis secretory protein Li 271 or mitochondrial ATP synthetase. This antibody has been extensively validated for western blotting using whole cell lysates.

ATP5B Antibody with Control Lysate

ATP5B antibody recognizes mitochondrial ATP synthase subunit beta, also known as mitochondrial epididymis secretory protein Li 271 or mitochondrial ATP synthetase. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI1F7
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00114 WB 100 µl
VMA00114KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human ATP5B antibody recognizes ATP5B, also known as ATP synthase subunit beta, mitochondrial, epididymis secretory protein Li 271, mitochondrial ATP synthase beta subunit and mitochondrial ATP synthetase, beta subunit.

Encoded by the ATP5B gene, ATP synthase subunit beta is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5B encodes the beta subunit of the catalytic core (provided by RefSeq, Jul 2008).

Mouse anti Human ATP5B antibody detects a band of 57 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ATP5B detects a band of approximately 57 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human ATP5B (NP_001677) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P06576 Related reagents
Entrez Gene
ATP5B Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0046961 proton-transporting ATPase activity, rotational mechanism
GO:0001525 angiogenesis
GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism
GO:0005524 ATP binding
GO:0005754 mitochondrial proton-transporting ATP synthase, catalytic core
GO:0022904 respiratory electron transport chain
GO:0008553 hydrogen-exporting ATPase activity, phosphorylative mechanism
GO:0009986 cell surface
GO:0042288 MHC class I protein binding
GO:0042645 mitochondrial nucleoid
GO:0042776 mitochondrial ATP synthesis coupled proton transport
GO:0043499 eukaryotic cell surface binding
GO:0051453 regulation of intracellular pH
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ATP5B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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