ATP Citrate Lyase antibody

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Mouse anti ATP Citrate Lyase

ATP-citrate lyase antibody recognizes the ATP-citrate lyase, also known as ATP-citrate (pro-S-)-lyase, ATP-citrate synthase or citrate cleavage enzyme. This antibody has been extensively validated for western blotting using whole cell lysates.

ATP Citrate Lyase Antibody with Control Lysate

ATP-citrate lyase antibody recognizes the ATP-citrate lyase, also known as ATP-citrate (pro-S-)-lyase, ATP-citrate synthase or citrate cleavage enzyme. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00116 WB 100 µl
VMA00116KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human ATP citrate lyase antibody recognizes ATP citrate lyase, also known as ATP citrate synthase, ATP-citrate (pro-S-)-lyase, ATP-citrate synthase and citrate cleavage enzyme.

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for ATP citrate lyase (provided by RefSeq, Jul 2008).

Mouse anti Human ATP citrate lyase antibody detects a band of 125 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment of human ATP Citrate Lysate, amino acids 306-502, expressed in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P53396 Related reagents
Entrez Gene
ACLY Related reagents
GO Terms
GO:0005515 protein binding
GO:0003878 ATP citrate synthase activity
GO:0005829 cytosol
GO:0004775 succinate-CoA ligase (ADP-forming) activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006101 citrate metabolic process
GO:0006112 energy reserve metabolic process
GO:0006200 ATP catabolic process
GO:0008815 citrate (pro-3S)-lyase activity
GO:0009346 citrate lyase complex
GO:0015936 coenzyme A metabolic process
GO:0019432 triglyceride biosynthetic process
GO:0031325 positive regulation of cellular metabolic process
GO:0035338 long-chain fatty-acyl-CoA biosynthetic process
GO:0044262 cellular carbohydrate metabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ATP Citrate Lyase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti ATP Citrate Lyase detects a band of approximately 125 kDa in HeLa cell lysates.
Western Blotting
Anti ATP Citrate Lyase detects a band of approximately 125 kDa in HeLa cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB