ATG3 antibody | OTI3C6

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Mouse anti ATG3

Product Type
PrecisionAb Monoclonal
Clone
OTI3C6
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00280
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human ATG3 antibody recognizes the ubiquitin-like-conjugating enzyme ATG3, also known as 2610016C12Rik, ATG3 autophagy related 3 homolog, autophagy-related protein 3, hApg3, ubiquitin-like-conjugating enzyme ATG3.

A product of the ATG3 gene, ubiquitin-like-conjugating enzyme ATG3 is a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of ATG3 is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2013).

Mouse anti Human ATG3 antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ATG3 detects a band of approximately 40 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human ATG3 (NP_071933) produced in HEK293T cells

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9NT62 Related reagents
Entrez Gene
ATG3 Related reagents
GO Terms
GO:0000045 autophagic vacuole assembly
GO:0000153 cytoplasmic ubiquitin ligase complex
GO:0005829 cytosol
GO:0016567 protein ubiquitination
GO:0006612 protein targeting to membrane
GO:0019899 enzyme binding
GO:0019776 Atg8 ligase activity
GO:0019777 Atg12 ligase activity
GO:0043653 mitochondrial fragmentation involved in apoptosis
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ATG3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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