ATF1 antibody | AB01/1D8

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Mouse anti ATF1

Product Type
PrecisionAb Monoclonal
Clone
AB01/1D8
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00798
Datasheet
WB
SDS
100 µl loader

Mouse anti ATF1 antibody recognizes cyclic AMP-dependent transcription factor ATF-1, also known as TREB36.

ATF1 is a basic region-leucine zipper transcription factor belonging to the cAMP response element-binding protein family. In embryonic stem cells (ESCs), ATF1 is a regulator of pluripotency and ATF1 knockdown has been shown to up-regulate neuroectoderm genes (Yang et al. 2019). Gene fusions between ATF1 and EWSR1 have been described in a range of neoplasms (Thway and Fisher 2012). ATF1 functions as an oncogene in colorectal cancer and facilitates proliferation by affecting apoptosis (Tian et al. 2019).

Product Details

Target Species
Human
Western Blotting
Mouse anti ATF1 antibody detects a band of approximately 37 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-271 of human ATF1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P18846
Entrez Gene
ATF1
GO Terms
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0008063 Toll signaling pathway
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051403 stress-activated MAPK cascade
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of ATF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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