ARNTL antibody | OTI3G9

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Mouse anti ARNTL

ARNTL antibody recognizes aryl hydrocarbon receptor nuclear translocator-like protein 1, also known as ARNT-like protein 1, brain and muscle, PAS domain-containing protein 3, bHLH-PAS protein JAP3, or basic-helix-loop-helix-PAS orphan MOP3.

ARNTL Antibody with Control Lysate

ARNTL antibody recognizes aryl hydrocarbon receptor nuclear translocator-like protein 1, also known as ARNT-like protein 1, brain and muscle, PAS domain-containing protein 3, bHLH-PAS protein JAP3, or basic-helix-loop-helix-PAS orphan MOP3.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI3G9
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00255 WB 100 µl
VMA00255KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human ARNTL antibody recognizes the aryl hydrocarbon receptor nuclear translocator-like protein 1, also known as ARNT-like protein 1, brain and muscle, PAS domain-containing protein 3, aryl hydrocarbon receptor nuclear translocator-like protein 1, bHLH-PAS protein JAP3, basic-helix-loop-helix-PAS orphan MOP3, basic-helix-loop-helix-PAS protein MOP3, brain and muscle ARNT-like 1, class E basic helix-loop-helix protein 5, member of PAS protein 3 and member of PAS superfamily 3.

Encoded by ARNTL gene, the aryl hydrocarbon receptor nuclear translocator-like protein 1 is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating ARNTL and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for ARNTL (provided by RefSeq, Jul 2008).

Mouse anti Human ARNTL antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ARTNL detects a band of approximately 78 kDa in NIH/3T3 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human ARNTL produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg NIH/3T3 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
O00327 Related reagents
Entrez Gene
ARNTL Related reagents
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ARNTL antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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