ARAF antibody | OTI2G4

Mouse anti ARAF

Product Type
PrecisionAb Monoclonal
Clone
OTI2G4
Isotype
IgG1
Format
Purified
Specificity
ARAF

Product Code Applications Pack Size List Price Your Price Qty
VMA00368
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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Mouse anti Human ARAF antibody recognizes the serine/threonine-protein kinase A-Raf, also known as oncogene ARAF1, Ras-binding protein DA-Raf and v-raf murine sarcoma 3611 viral oncogene homolog 1.

This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for ARAF (provided by RefSeq, Jan 2012).

Mouse anti Human ARAF antibody detects a band of 68 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti ARAF detects a band of approximately 68 kDa in HEK293 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein fragment corresponding to aa 145-365 of human ARAF (NP_001645) produced in E.coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P10398
Entrez Gene
ARAF
GO Terms
GO:0005515 protein binding
GO:0004674 protein serine/threonine kinase activity
GO:0005057 receptor signaling protein activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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