ARAF antibody | OTI2G4
Mouse anti ARAF
Mouse anti human ARAF antibody recognizes serine/threonine-protein kinase A-Raf, also known as oncogene ARAF1, Ras-binding protein DA-Raf, proto-oncogene A-Raf-1 or v-raf murine sarcoma 3611 viral oncogene homolog 1. This antibody has been validated for western blotting using whole cell lysates.
ARAF Antibody with Control Lysate
Mouse anti human ARAF antibody recognizes serine/threonine-protein kinase A-Raf, also known as oncogene ARAF1, Ras-binding protein DA-Raf, proto-oncogene A-Raf-1 or v-raf murine sarcoma 3611 viral oncogene homolog 1. This antibody has been extensively validated for western blotting.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI2G4
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00368 | WB | 100 µl | ||||
| VMA00368KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human ARAF antibody recognizes the serine/threonine-protein kinase A-Raf, also known as oncogene ARAF1, Ras-binding protein DA-Raf and v-raf murine sarcoma 3611 viral oncogene homolog 1.
This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for ARAF (provided by RefSeq, Jan 2012).
Mouse anti Human ARAF antibody detects a band of 68 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for ARAF (provided by RefSeq, Jan 2012).
Mouse anti Human ARAF antibody detects a band of 68 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti ARAF detects a band of approximately 68 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Recombinant protein fragment corresponding to aa 145-365 of human ARAF (NP_001645) produced in E.coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P10398 Related reagents
- Entrez Gene
- ARAF Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0004674 protein serine/threonine kinase activity
- GO:0005057 receptor signaling protein activity
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0006468 protein phosphorylation
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of ARAF antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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