ARAF antibody | OTI2G4

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Mouse anti ARAF

Mouse anti human ARAF antibody recognizes serine/threonine-protein kinase A-Raf, also known as oncogene ARAF1, Ras-binding protein DA-Raf, proto-oncogene A-Raf-1 or v-raf murine sarcoma 3611 viral oncogene homolog 1. This antibody has been validated for western blotting using whole cell lysates.

ARAF Antibody with Control Lysate

Mouse anti human ARAF antibody recognizes serine/threonine-protein kinase A-Raf, also known as oncogene ARAF1, Ras-binding protein DA-Raf, proto-oncogene A-Raf-1 or v-raf murine sarcoma 3611 viral oncogene homolog 1. This antibody has been extensively validated for western blotting.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI2G4
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00368 WB 100 µl
VMA00368KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human ARAF antibody recognizes the serine/threonine-protein kinase A-Raf, also known as oncogene ARAF1, Ras-binding protein DA-Raf and v-raf murine sarcoma 3611 viral oncogene homolog 1.

This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for ARAF (provided by RefSeq, Jan 2012).

Mouse anti Human ARAF antibody detects a band of 68 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein fragment corresponding to aa 145-365 of human ARAF (NP_001645) produced in E.coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P10398 Related reagents
Entrez Gene
ARAF Related reagents
GO Terms
GO:0005515 protein binding
GO:0004674 protein serine/threonine kinase activity
GO:0005057 receptor signaling protein activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of ARAF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti ARAF detects a band of approximately 68 kDa in HEK293 cell lysates
Western Blotting
Anti ARAF detects a band of approximately 68 kDa in HEK293 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB