Apolipoprotein A1 antibody | G2

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Mouse anti Human Apolipoprotein A1

Product Type
Monoclonal Antibody
Clone
G2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0650-0050 C E WB 0.5 mg
Mouse anti Human Apolipoprotein A1, clone G2 recognizes Apolipoprotein A-1 (also known as Apo-A1) , a lipid-binding protein which enables the transport of dietary lipids for storage, metabolism and secretion. Apo-A1 plays an important part in the removal of cholesterol from cells.

Mouse anti Human Apolipoprotein A1, clone G2 reacts with both free human Apo-A1 and High Density Lipoprotein (HDL) bearing Apo-A1, but does not cross-react with ApoE, ApoB or Albumin.

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
0.01M Sodium Phosphate
0.01M Sodium Borate
0.15M Sodium Chloride
Preservative Stabilisers
1% Dextran
1% Mannitol
Immunogen
Native Apolipoprotein-A1 from human plasma

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P02647 Related reagents
Entrez Gene
APOA1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001540 beta-amyloid binding
GO:0002576 platelet degranulation
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0005543 phospholipid binding
GO:0005788 endoplasmic reticulum lumen
GO:0030141 stored secretory granule
GO:0030139 endocytic vesicle
GO:0006656 phosphatidylcholine biosynthetic process
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0042802 identical protein binding
GO:0010873 positive regulation of cholesterol esterification
GO:0010903 negative regulation of very-low-density lipoprotein particle remodeling
GO:0015485 cholesterol binding
GO:0050821 protein stabilization
GO:0017127 cholesterol transporter activity
GO:0019899 enzyme binding
GO:0030168 platelet activation
GO:0032488 Cdc42 protein signal transduction
GO:0033344 cholesterol efflux
GO:0033700 phospholipid efflux
GO:0034191 apolipoprotein A-I receptor binding
GO:0034361 very-low-density lipoprotein particle
GO:0034366 spherical high-density lipoprotein particle
GO:0034375 high-density lipoprotein particle remodeling
GO:0034380 high-density lipoprotein particle assembly
GO:0034384 high-density lipoprotein particle clearance
GO:0042632 cholesterol homeostasis
GO:0043691 reverse cholesterol transport
GO:0050713 negative regulation of interleukin-1 beta secretion
GO:0051345 positive regulation of hydrolase activity
GO:0060228 phosphatidylcholine-sterol O-acyltransferase activator activity
GO:0070508 cholesterol import
GO:0070653 high-density lipoprotein particle receptor binding
Regulatory
For research purposes only

Applications of Apolipoprotein A1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000
Immunohistology - Frozen 1/40
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

ELISA
This antibody is suitable for coating microtitre plates in a sandwich ELISA using catalogue number 0650-0190 for detection.

Product Specific References

References for Apolipoprotein A1 antibody

  1. Derbali, H. et al. (2010) Increased biglycan in aortic valve stenosis leads to the overexpression of phospholipid transfer protein via Toll-like receptor 2.
    Am J Pathol. 176: 2638-45.
  2. Mogilenko, D.A. et al. (2012) Endogenous apolipoprotein A-I stabilizes ATP-binding cassette transporter A1 and modulates Toll-like receptor 4 signaling in human macrophages.
    FASEB J. 26: 2019-30.
  3. Shavva VS et al. (2016) PPARγ Represses Apolipoprotein A-I Gene but Impedes TNFα-Mediated ApoA-I Downregulation in HepG2 Cells.
    J Cell Biochem. : .
  4. Pingitore, P. et al. (2016) Identification and characterization of two novel mutations in the LPL gene causing Type I hyperlipoproteinemia
    Journal of Clinical Lipidology. 10 Mar [Epub ahead of print]