APOBEC3G antibody

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Mouse anti APOBEC3G

Mouse anti human APOBEC3G antibody recognizes APOBEC-related cytidine deaminase, also known as APOBEC-related protein 9, DNA dC->dU editing enzyme, deoxycytidine deaminase or phorbolin-like protein MDS019. This antibody has been extensively validated for western blotting using whole cell lysates.

APOBEC3G Antibody with Control Lysate

Mouse anti human APOBEC3G antibody recognizes APOBEC-related cytidine deaminase, also known as APOBEC-related protein 9, DNA dC->dU editing enzyme, apolipoprotein B editing enzyme catalytic polypeptide-like 3G, deoxycytidine deaminase or phorbolin-like protein MDS019.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00418 WB 100 µl
VMA00418KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human APOBEC3G antibody recognizes APOBEC3G, also known as APOBEC-related cytidine deaminase, apolipoprotein B mRNA-editing enzyme catalytic polypeptide 3G, deoxycytidine deaminase and phorbolin-like protein MDS019.

The APOBEC3G gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by APOBEC3G has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity (provided by RefSeq, Jul 2008).

Mouse anti Human APOBEC3G antibody detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti APOBEC3G detects a band of approximately 46 kDa in HEK293 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E.coli-derived recombinant protein corresponding to aa 60-330 of human APOBEC3G
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9HC16 Related reagents
Entrez Gene
APOBEC3G Related reagents
GO Terms
GO:0002230 positive regulation of defense response to virus by host
GO:0003723 RNA binding
GO:0009615 response to virus
GO:0004126 cytidine deaminase activity
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0006410 transcription, RNA-dependent
GO:0008270 zinc ion binding
GO:0008829 dCTP deaminase activity
GO:0016032 viral reproduction
GO:0016553 base conversion or substitution editing
GO:0030895 apolipoprotein B mRNA editing enzyme complex
GO:0042803 protein homodimerization activity
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
GO:0045869 negative regulation of retroviral genome replication
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of APOBEC3G antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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