Find out more about A.I.den - our SearchBot

APEX1 antibody | CPTC15

ISO 9001 Quality Assurance Manufacturing | Print
KO Validated
Anti APEX1 Antibody, clone CPTC15 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti APEX1 Antibody, clone CPTC15 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti APEX1 Antibody, clone CPTC15 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti APEX1 Antibody, clone CPTC15 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with APEX1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 1 second exposure. Arrow points to APEX1 (molecular weight 36 kDa)
Anti APEX1 Antibody, clone CPTC15 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of APEX1 CRISPR knockout HEK293 (APEX1 KO) and wild type HEK293 (WT) whole cell lysates probed with (A) Mouse anti APEX1 antibody (VMA00561) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 12 second and (B) 30 second exposure.
Anti APEX1 Antibody, clone CPTC15 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of APEX1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Filter by Application:

WB

Mouse anti APEX1

Product Type
PrecisionAb Monoclonal
Clone
CPTC15
Isotype
IgG1
Format
Purified
Specificity
APEX1

Quick Links:

Antibody Quality and Performance Guarantee Antibody Quality and Performance GuaranteeAntibody Quality and
Performance Guarantee
Unrivaled technical support Unrivalled technical supportUnrivaled
Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
VMA00561
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human APEX1 antibody recognizes the apurinic/apyrimidinic endodeoxyribonuclease 1, also known as AP endonuclease class I, AP lyase or protein REF-1.

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. The APEX1 gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein (provided by RefSeq, Jul 2008).

Mouse anti Human APEX1 antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti APEX1 detects a band of approximately 36 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant protein of human APEX1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P27695
Entrez Gene
APEX1
GO Terms
GO:0005515 protein binding
GO:0003684 damaged DNA binding
GO:0003713 transcription coactivator activity
GO:0003714 transcription corepressor activity
GO:0003723 RNA binding
GO:0003906 DNA-(apurinic or apyrimidinic site) lyase activity
GO:0005739 mitochondrion
GO:0004523 ribonuclease H activity
GO:0004528 phosphodiesterase I activity
GO:0004844 uracil DNA N-glycosylase activity
GO:0005783 endoplasmic reticulum
GO:0046872 metal ion binding
GO:0005840 ribosome
GO:0005730 nucleolus
GO:0005813 centrosome
GO:0016491 oxidoreductase activity
GO:0006284 base-excision repair
GO:0006310 DNA recombination
GO:0008408 3'-5' exonuclease activity
GO:0016607 nuclear speck
GO:0016890 site-specific endodeoxyribonuclease activity, specific for altered base
GO:0031490 chromatin DNA binding
GO:0043488 regulation of mRNA stability
GO:0045739 positive regulation of DNA repair
GO:0045768 positive regulation of anti-apoptosis
GO:0048471 perinuclear region of cytoplasm
View more products with APEX1 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products