APC2 antibody | OTI1B11
Mouse anti APC2
APC2 antibody recognizes anaphase promoting complex subunit 2 also known as cyclosome subunit 2.
APC2 Antibody with Control Lysate
APC2 antibody recognizes anaphase promoting complex subunit 2 also known as cyclosome subunit 2.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI1B11
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00604 | WB | 100 µl | ||||
| VMA00604KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human APC2 antibody recognizes the anaphase promoting complex subunit 2 also known as cyclosome subunit 2.
A large protein complex, termed the anaphase-promoting complex (APC) or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation (provided by RefSeq, Jul 2008).
Mouse anti Human APC2 antibody detects a band of 100kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
A large protein complex, termed the anaphase-promoting complex (APC) or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation (provided by RefSeq, Jul 2008).
Mouse anti Human APC2 antibody detects a band of 100kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti APC2 antibody detects a band of approximately 100 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol - Immunogen
- HEK293-derived full length recombinant human APC2
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q9UJX6 Related reagents
- Entrez Gene
- ANAPC2 Related reagents
- GO Terms
- GO:0000079 regulation of cyclin-dependent protein kinase activity
- GO:0005829 cytosol
- GO:0004842 ubiquitin-protein ligase activity
- GO:0005654 nucleoplasm
- GO:0005680 anaphase-promoting complex
- GO:0007067 mitosis
- GO:0007094 mitotic cell cycle spindle assembly checkpoint
- GO:0008054 cyclin catabolic process
- GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- GO:0031461 cullin-RING ubiquitin ligase complex
- GO:0031625 ubiquitin protein ligase binding
- GO:0051301 cell division
- GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
- GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
- GO:0070979 protein K11-linked ubiquitination
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of APC2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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