Annexin V antibody | VAA-33
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Mouse anti Human Annexin V antibody, clone VAA-33 recognizes human Annexin V, a phospholipid binding protein which binds with high affinity and selectivity to phosphatidylserine (PS), in the presence of calcium.
The translocation of PS from the cytosolic leaflet to the outer leaflet of the plasma membrane, due to a loss in plasma membrane asymmetry during apoptosis, acts as an in vivo signal for the removal of the dying cell by phagocytosis. Detection of PS using Annexin V therefore provides a reliable diagnostic tool for the indication of early programmed cell death.
Annexin V has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Annexin V, are associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc).
Mouse anti Human Annexin V antibody, clone VAA-33 has been reported to work in Western blotting applications (Wedher et al. 2009).
The translocation of PS from the cytosolic leaflet to the outer leaflet of the plasma membrane, due to a loss in plasma membrane asymmetry during apoptosis, acts as an in vivo signal for the removal of the dying cell by phagocytosis. Detection of PS using Annexin V therefore provides a reliable diagnostic tool for the indication of early programmed cell death.
Annexin V has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Annexin V, are associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc).
Mouse anti Human Annexin V antibody, clone VAA-33 has been reported to work in Western blotting applications (Wedher et al. 2009).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide (NaN3) 1% Bovine Serum Albumin - Preservative Stabilisers
0.02% Sodium Azide (NaN3) 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Recombinant Annexin V.
- Approx. Protein Concentrations
- IgG concentration 0.2 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- Please see label for expiry date.
- Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P08758 Related reagents
- Entrez Gene
- ANXA5 Related reagents
- GO Terms
- GO:0005509 calcium ion binding
- GO:0007596 blood coagulation
- GO:0004859 phospholipase inhibitor activity
- GO:0007165 signal transduction
- GO:0005544 calcium-dependent phospholipid binding
- GO:0005737 cytoplasm
- GO:0006916 anti-apoptosis
- GO:0050819 negative regulation of coagulation
- Regulatory
- For research purposes only
Applications of Annexin V antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Flow Cytometry | Neat | ||
| ELISA | |||
| Flow Cytometry |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:FITC | MCA929F | 100 Tests | F | ||
| Mouse IgG2a Negative Control | MCA929 | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Annexin V:Biotin Assay Kit | ANNEX100B | 100 Tests | F | ||
| Annexin V:FITC Assay Kit | ANNEX100F | 100 Tests | F | ||
| Annexin V:FITC Assay Kit | ANNEX300F | 300 Tests | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Mouse anti Human Annexin V | MCA2712 | 0.1 mg | E F | ||
| Mouse anti Human Annexin V:FITC | MCA2712F | 100 Tests | F | ||
| Annexin V:Biotin Assay Kit | ANNEX100B | 100 Tests | F | ||
| Annexin V:FITC Assay Kit | ANNEX100F | 100 Tests | F | ||
| Annexin V:FITC Assay Kit | ANNEX300F | 300 Tests | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Mouse anti Human Annexin V | MCA2712 | 0.1 mg | E F | ||
| Mouse anti Human Annexin V:Biotin | MCA2712B | 100 Tests/1ml | F | ||
| Annexin V:Biotin Assay Kit | ANNEX100B | 100 Tests | F | ||
| Annexin V:FITC Assay Kit | ANNEX100F | 100 Tests | F | ||
| Annexin V:FITC Assay Kit | ANNEX300F | 300 Tests | F | ||
| Mouse anti Human Annexin V:Biotin | MCA2712B | 100 Tests/1ml | F | ||
| Mouse anti Human Annexin V:FITC | MCA2712F | 100 Tests | F |
Product Specific References
Further Reading
-
Gerke, V. & Moss, S.E. (2002) Annexins: from structure to function.
Physiol Rev. 82 (2): 331-71.
