Annexin V antibody | VAA-33

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Mouse anti Human Annexin V:Biotin

Mouse anti Human Annexin V:FITC

Mouse anti Human Annexin V

Product Type
Monoclonal Antibody
Clone
VAA-33
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2712B F 100 Tests/1ml
MCA2712F F 100 Tests
MCA2712 E F 0.1 mg
Mouse anti Human Annexin V antibody, clone VAA-33 recognizes human Annexin V, a phospholipid binding protein which binds with high affinity and selectivity to phosphatidylserine (PS), in the presence of calcium.

The translocation of PS from the cytosolic leaflet to the outer leaflet of the plasma membrane, due to a loss in plasma membrane asymmetry during apoptosis, acts as an in vivo signal for the removal of the dying cell by phagocytosis. Detection of PS using Annexin V therefore provides a reliable diagnostic tool for the indication of early programmed cell death.

Annexin V has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Annexin V, are associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc).

Mouse anti Human Annexin V antibody, clone VAA-33 has been reported to work in Western blotting applications (Wedher et al. 2009).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Recombinant Annexin V.
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
Please see label for expiry date.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P08758 Related reagents
Entrez Gene
ANXA5 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0007596 blood coagulation
GO:0004859 phospholipase inhibitor activity
GO:0007165 signal transduction
GO:0005544 calcium-dependent phospholipid binding
GO:0005737 cytoplasm
GO:0006916 anti-apoptosis
GO:0050819 negative regulation of coagulation
Regulatory
For research purposes only

Applications of Annexin V antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Flow Cytometry Neat
ELISA
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F 100 Tests F
Mouse IgG2a Negative Control MCA929 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Annexin V:Biotin Assay Kit ANNEX100B 100 Tests F
Annexin V:FITC Assay Kit ANNEX100F 100 Tests F
Annexin V:FITC Assay Kit ANNEX300F 300 Tests F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Mouse anti Human Annexin V MCA2712 0.1 mg E F
Mouse anti Human Annexin V:FITC MCA2712F 100 Tests F
Annexin V:Biotin Assay Kit ANNEX100B 100 Tests F
Annexin V:FITC Assay Kit ANNEX100F 100 Tests F
Annexin V:FITC Assay Kit ANNEX300F 300 Tests F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Mouse anti Human Annexin V MCA2712 0.1 mg E F
Mouse anti Human Annexin V:Biotin MCA2712B 100 Tests/1ml F
Annexin V:Biotin Assay Kit ANNEX100B 100 Tests F
Annexin V:FITC Assay Kit ANNEX100F 100 Tests F
Annexin V:FITC Assay Kit ANNEX300F 300 Tests F
Mouse anti Human Annexin V:Biotin MCA2712B 100 Tests/1ml F
Mouse anti Human Annexin V:FITC MCA2712F 100 Tests F

Product Specific References

Further Reading

  1. Gerke, V. & Moss, S.E. (2002) Annexins: from structure to function.
    Physiol Rev. 82 (2): 331-71.