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Annexin V antibody | VAA-33

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Mouse anti Human Annexin V:Biotin

Product Type
Monoclonal Antibody
Clone
VAA-33
Isotype
IgG2a
Specificity
Annexin V

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MCA2712B
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Mouse anti Human annexin V antibody, clone VAA-33 recognizes human Annexin V, a phospholipid binding protein which binds with high affinity and selectivity to phosphatidylserine (PS), in the presence of calcium.

The translocation of PS from the cytosolic leaflet to the outer leaflet of the plasma membrane, due to a loss in plasma membrane asymmetry during apoptosis, acts as an in vivo signal for the removal of the dying cell by phagocytosis. Detection of PS using Annexin V therefore provides a reliable diagnostic tool for the indication of early programmed cell death.

Annexin V has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Annexin V, are associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc).

Mouse anti Human annexin V antibody, clone VAA-33 has been reported to work in Western blotting applications (Wedher et al. 2009).

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Immunogen
Recombinant Annexin V.
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock
Annexin V:FITC Assay Kit ANNEX300F F 300 Tests
List Price Your Price
Description Annexin V:FITC Assay Kit

Further Reading

  1. Gerke, V. & Moss, S.E. (2002) Annexins: from structure to function.
    Physiol Rev. 82 (2): 331-71.

RRID
AB_915142
UniProt
P08758
Entrez Gene
ANXA5
GO Terms
GO:0005509 calcium ion binding
GO:0007596 blood coagulation
GO:0004859 phospholipase inhibitor activity
GO:0007165 signal transduction
GO:0005544 calcium-dependent phospholipid binding
GO:0005737 cytoplasm
GO:0006916 anti-apoptosis
GO:0050819 negative regulation of coagulation
View more products with ANNEXIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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