Annexin III antibody | OTI1F11

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Mouse anti Annexin III

Annexin III antibody recognizes annexin III, also known as ANXA3, 35-alpha calcimedin, PAP-III, or lipocortin III

Annexin III Antibody with Control Lysate

Annexin III antibody recognizes annexin III, also known as ANXA3, 35-alpha calcimedin, PAP-III, or lipocortin III

Product Type
PrecisionAb™ Monoclonal
Clone
OTI1F11
Isotype
IgG2b
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00724 WB 100 µl
VMA00724KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human annexin III antibody recognizes annexin III, also known as ANXA3, 35-alpha calcimedin, PAP-III and lipocortin III

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation (provided by RefSeq, Jul 2008).

Mouse anti Human annexin III antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti annexin III detects a band of approximately 36 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen
HEK293-derived full length recombinant protein of human annexin III
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P12429 Related reagents
Entrez Gene
ANXA3 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005509 calcium ion binding
GO:0042742 defense response to bacterium
GO:0005544 calcium-dependent phospholipid binding
GO:0006909 phagocytosis
GO:0010595 positive regulation of endothelial cell migration
GO:0019834 phospholipase A2 inhibitor activity
GO:0030670 phagocytic vesicle membrane
GO:0042581 specific granule
GO:0043312 neutrophil degranulation
GO:0045766 positive regulation of angiogenesis
GO:0051091 positive regulation of transcription factor activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Annexin III antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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