Angiogenin antibody | MANG-1

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Mouse anti Human Angiogenin

Product Type
Monoclonal Antibody
Clone
MANG-1
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0555-5008 C E P* 0.15 mg
Mouse anti Human Angiogenin anibody, clone MANG-1 is a monoclonal antibody specific for angiogenin, a potent angiogenesis inducing polypeptide of approximately 16.5kDa responsible for inducing vascularization in both normal and malignant tissues through activation of vascular endothelium and smooth muscle cells ant triggering a number of processes including Cell migration and proliferation (Reviewed in Gao et al.2008) Angiogenin, also known as ribonuclease 5 demonstrates tRNA specific ribonuclease activity (Saxena et al. 1992), this in turn is regulated by binding to the ribonuclease inhibitor RNH1 (Tuefel et al. 2003) . Angiogenin exerts its vascular stimulation potential through binding to cytoplasmic actin on the surface of endothelial cells, followed by endocytosis and subsequent translocation to the nucleus

Mouse anti Angiogenin, clone MANG1 stains single cells on human tonsil sections and endothelial cells in human terminal placenta. MANG-1 also demonstrates intense staining on carcinoma cells and of endothelial cells in intratumoral vessels (Marioni et al. 2010)

Product Details

Target Species
Human
Product Form
Tissue Culture Supernatant - lyophilised
Reconstitution
500 µl sterile deionised water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Thiomersal
1%Bovine Serum Albumin
Immunogen
Recombinant human angiogenin
Approx. Protein Concentrations
IgG concentration 0.3 mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P03950 Related reagents
Entrez Gene
ANG Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0001541 ovarian follicle development
GO:0001556 oocyte maturation
GO:0001666 response to hypoxia
GO:0001890 placenta development
GO:0001938 positive regulation of endothelial cell proliferation
GO:0003677 DNA binding
GO:0003779 actin binding
GO:0005102 receptor binding
GO:0004522 pancreatic ribonuclease activity
GO:0005507 copper ion binding
GO:0005605 basal lamina
GO:0005615 extracellular space
GO:0005730 nucleolus
GO:0006651 diacylglycerol biosynthetic process
GO:0007154 cell communication
GO:0007202 activation of phospholipase C activity
GO:0008201 heparin binding
GO:0008219 cell death
GO:0030041 actin filament polymerization
GO:0009303 rRNA transcription
GO:0009725 response to hormone stimulus
GO:0016477 cell migration
GO:0017148 negative regulation of translation
GO:0019843 rRNA binding
GO:0030426 growth cone
GO:0032148 activation of protein kinase B activity
GO:0032311 angiogenin-PRI complex
GO:0032431 activation of phospholipase A2 activity
GO:0042277 peptide binding
GO:0042327 positive regulation of phosphorylation
GO:0042592 homeostatic process
GO:0043025 neuronal cell body
GO:0048662 negative regulation of smooth muscle cell proliferation
GO:0050714 positive regulation of protein secretion
Regulatory
For research purposes only

Applications of Angiogenin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen 1/60
Immunohistology - Paraffin 1 1/30
  1. 1Proteinase K pretreatment for antigen retrieval is recommended for use with paraffin sections.See Marioni et al. 2011
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Human tonsil

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for Angiogenin antibody

  1. Marioni, G. et al. (2010) Neoangiogenesis in laryngeal carcinoma: angiogenin and CD105 expression is related to carcinoma recurrence rate and disease-free survival.
    Histopathology. 57: 535-43.
  2. Marioni, G. et al. (2013) The role of angiogenin in pT1-T2 tongue carcinoma neo-angiogenesis and cell proliferation: an exploratory study.
    J Oral Pathol Med. 42: 606-11.
  3. Marioni, G. et al. (2011) Laryngeal carcinoma prognosis after postoperative radiotherapy correlates with CD105 expression, but not with angiogenin or EGFR expression.
    Eur Arch Otorhinolaryngol. 268: 1779-87.
  4. Seilhean, D. et al. (2009) Accumulation of TDP-43 and alpha-actin in an amyotrophic lateral sclerosis patient with the K17I ANG mutation.
    Acta Neuropathol. 118: 561-73.
  5. Marioni, G. et al. (2014) A panel of biomarkers for predicting response to postoperative RT for laryngeal cancer?
    Am J Otolaryngol. pii: S0196-0709(14)00137-9.
  6. Pan, S.C. et al. (2012) Angiogenin expression in burn blister fluid: Implications for its role in burn wound neovascularization.
    Wound Repair Regen. 20: 731-9.
  7. Kirby, J. et al. (2013) Lack of unique neuropathology in amyotrophic lateral sclerosis associated with p.K54E angiogenin (ANG) mutation.
    Neuropathol Appl Neurobiol. 2013 Aug;39(5): 562-71.
  8. Marioni, G. et al. (2013) Indications for postoperative radiotherapy in laryngeal carcinoma: A panel of tumor tissue markers for predicting locoregional recurrence in surgically treated carcinoma. A pilot study.
    Head Neck. 36: 1534-40.
  9. Lovato, A. et al. (2015) A Higher Angiogenin Expression is Associated With a Nonnuclear Maspin Location in Laryngeal Carcinoma
    Clinical and Experimental Otorhinolaryngology. 8 (3): 268.

Further Reading

  1. Fett, J. W. et al. (1985) Isolation and characterization of angiogenin, an angiogenic protein from human carcinoma cells.
    Biochemistry. 24: 5480-6.