Amylase antibody | 2D4
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P ResetMouse anti Human Amylase (Salivary):Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 2D4
- Isotype
- IgG2a
- Specificity
- Amylase
- Region
- (SALIVARY)
Antibody Quality and Performance Guarantee
| Mouse anti Human salivary amylase antibody recognizes human Alpha-amylase 1, also known as salivary amylase, 1,4-alpha-D-glucan glucanohydrolase 1 or Salivary alpha-amylase. Salivary amylase is a 496 amino acid ~58 kDa secreted protein and member of the glycosyl hydrolase 13 family. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 172 - 246 of human AMY1A.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 |  |
0.1 | 10 ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Amylase antibody
-
Jain, S. et al. (2013) Amylase α-1A (AMY1A): a novel immunohistochemical marker to differentiate chromophobe renal cell carcinoma from benign oncocytoma.
Am J Surg Pathol. 37 (12): 1824-30.
- RRID
- AB_2242136
- UniProt
- P04745
- Entrez Gene
- AMY1C
- GO Terms
- GO:0005515 protein binding
- GO:0004556 alpha-amylase activity
- GO:0005576 extracellular region
- GO:0046872 metal ion binding
- GO:0005975 carbohydrate metabolic process
- GO:0007586 digestion
MCA2928Z
250815
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