Amylase antibody | 2D4

Mouse anti Human Amylase (Salivary):Preservative Free

Product Type
Monoclonal Antibody
Clone
2D4
Isotype
IgG2a
Specificity
Amylase
Region
(SALIVARY)

Product Code Applications Pack Size List Price Your Price Qty
MCA2928Z
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human salivary amylase antibody recognizes human Alpha-amylase 1, also known as salivary amylase, 1,4-alpha-D-glucan glucanohydrolase 1 or Salivary alpha-amylase. Salivary amylase is a 496 amino acid ~58 kDa secreted protein and member of the glycosyl hydrolase 13 family.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 172 - 246 of human AMY1A.
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 0.1 10 ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for Amylase antibody

  1. Jain, S. et al. (2013) Amylase α-1A (AMY1A): a novel immunohistochemical marker to differentiate chromophobe renal cell carcinoma from benign oncocytoma.
    Am J Surg Pathol. 37 (12): 1824-30.

RRID
AB_2242136
UniProt
P04745
Entrez Gene
AMY1C
GO Terms
GO:0005515 protein binding
GO:0004556 alpha-amylase activity
GO:0005576 extracellular region
GO:0046872 metal ion binding
GO:0005975 carbohydrate metabolic process
GO:0007586 digestion
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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