AMPK Alpha 1 antibody

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Mouse anti AMPK Alpha 1

AMPK alpha 1 antibody recognizes 5'-amp-activated protein kinase catalytic subunit alpha-1, also known as 5'-AMP-activated protein kinase, catalytic alpha-1 chain, ACACA kinase, AMPK alpha 1, HMGCR kinase, or acetyl-CoA carboxylase kinase.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00246 WB 100 µl
Mouse anti Human AMPK alpha 1 antibody recognizes the 5'-AMP-activated protein kinase catalytic subunit alpha-1, also known as 5'-AMP-activated protein kinase catalytic subunit alpha-1, 5'-AMP-activated protein kinase, catalytic alpha-1 chain, ACACA kinase, AMP -activate kinase alpha 1 subunit, AMP-activated protein kinase, catalytic, alpha-1, AMPK alpha 1, AMPK subunit alpha-1, HMGCR kinase, acetyl-CoA carboxylase kinase, hydroxymethylglutaryl-CoA reductase kinase and tau-protein kinase PRKAA1.

Encoded by PRKAA1 gene, AMPK alpha 1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed (provided by RefSeq, Jul 2008).

Mouse anti Human AMPK alpha 1 antibody detects a band of 64 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti AMPK alpha 1 detects a band of approximately 64 kDa in C6 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment of human AMPK alpha 1 expressed in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q13131 Related reagents
Entrez Gene
PRKAA1 Related reagents
GO Terms
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0001666 response to hypoxia
GO:0005829 cytosol
GO:0004691 cAMP-dependent protein kinase activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0006633 fatty acid biosynthetic process
GO:0006695 cholesterol biosynthetic process
GO:0007050 cell cycle arrest
GO:0008286 insulin receptor signaling pathway
GO:0045542 positive regulation of cholesterol biosynthetic process
GO:0045768 positive regulation of anti-apoptosis
GO:0046318 negative regulation of glucosylceramide biosynthetic process
GO:0046320 regulation of fatty acid oxidation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of AMPK Alpha 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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