Aly antibody | AB01/1C6

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Mouse anti Aly

ALY antibody recognizes ALY, also known as ALYREF, Aly/REF export factor, THO complex 4 or bZIP enhancing factor.

Aly Antibody with Control Lysate

ALY antibody recognizes ALY, also known as ALYREF, Aly/REF export factor, THO complex 4 or bZIP enhancing factor.

Product Type
PrecisionAb™ Monoclonal
Clone
AB01/1C6
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00616 WB 100 µl
VMA00616KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human ALY antibody recognizes ALY also known as ALYREF, Aly/REF export factor, THO complex 4 or bZIP enhancing factor.

The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins (provided by RefSeq, Jul 2008).

Mouse anti Human ALY antibody detects a band of 26 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti ALY antibody detects a band of approximately 26 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human ALY (aa 1-257)
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q86V81 Related reagents
Entrez Gene
THOC4 Related reagents
GO Terms
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0000346 transcription export complex
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0003723 RNA binding
GO:0005829 cytosol
GO:0005681 spliceosomal complex
GO:0006369 termination of RNA polymerase II transcription
GO:0016607 nuclear speck
GO:0031124 mRNA 3'-end processing
GO:0046784 intronless viral mRNA export from host nucleus
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Aly antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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