Alpha Fetoprotein antibody

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Anti Alpha Fetoprotein Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with alpha fetoprotein antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/50,000, STAR207P) and visualized on the ChemiDoc MP with 300 second exposure, Arrow points to alpha fetoprotein (molecular weight 69 kDa)

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Mouse anti Alpha Fetoprotein

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified
Specificity: Alpha Fetoprotein

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Mouse anti Human alpha fetoprotein antibody recognizes alpha fetoprotein (AFP), a 609 amino acid member of the ALB/AFP/VDB family containing 3 albumin domains. Human alpha fetoprotein, also known as alpha fetoglobulin is a plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to 16th week of gestation. It binds and transports various ligands such as bilirubin, fatty acids, steroids, heavy metals and various drugs (Aoyagi et al. 1979). After birth, plasma levels drop to trace amounts in healthy adults.

Elevated AFP levels are indicative of certain cancers, particularly hepatocellular carcinoma, cancer of the testes or ovaries or a malignant teratoma. During pregnancy increased levels of AFP may indicate neural tube defects such as spina bifida (Bredaki et al. 2012), while decreased levels could indicate Down’s syndrome (Norgaard-Pedersen et al. 1976).

Mouse Anti Human Alpha Fetoprotein antibody recognizes alpha fetoprotein as a band of ~69 kDa in HepG2 whole cell lysate by Western blotting under reducing conditions and does not cross-react with human albumin.

Target Species: Human
Western Blotting: Anti alpha fetoprotein antibody detects a band of approximately 69 kDa in HepG2 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Purified human alpha fetoprotein
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.