Alpha Fetoprotein antibody | 125.7A2

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Mouse anti Human Alpha Fetoprotein

Product Type
Monoclonal Antibody
Clone
125.7A2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5861G E 1 mg
Mouse anti Human alpha fetoprotein antibody, clone 125.7A2 is a monoclonal antibody recognizing alpha fetoprotein (AFP), a member of the albuminoid protein superfamily, along with albumin and vitamin D binding protein. Members of the albuminoid superfamily comprise molecules with three characteristic globular domains (Mizejewski 1995).

AFP is a ~70kD plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to 16th week of gestation. AFP acts as a transport protein for various ligands such as bilirubin, fatty acids, steroids and heavy metals. After birth, plasma AFP levels drop rapidly with only trace amounts remaining in healthy adults. Changes in plasma levels of AFP have been documented in a variety of cancers including hepatocellular cancer (Sato et al. 1993).

Mouse anti Human alpha fetoprotein antibody, clone 125.7A2 shows <0.1% reactivity with human serum albumin and IgG. Strong expression of AFP has been noted in the hepatic cell line HepG2.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

UniProt
P02771 Related reagents
Entrez Gene
AFP Related reagents
GO Terms
GO:0046872 metal ion binding
GO:0006810 transport
Regulatory
For research purposes only

Applications of Alpha Fetoprotein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5µg/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.