Alpha B Crystallin antibody | 2E8
Lane 1: Recombinant alpha a crystallin
Lane 2: Recombinant alpha b crystallin.
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WB ResetMouse anti Human Alpha B Crystallin
- Product Type
- Monoclonal Antibody
- Clone
- 2E8
- Isotype
- IgG2b
- Specificity
- Alpha B Crystallin
Antibody Quality and Performance Guarantee
| Mouse anti Human alpha B crystallin, clone 2E8 recognizes the basic (b) form of alpha crystallin, alpha B crystallin, a 20kDa water-soluble major structural protein and member of the heat shock protein family, designated HSPB5, which acts as a molecular chaperone and plays a role in the maintenance of ocular lens transparency/refractive index. Besides the involvement of both alpha A and alpha B crystallin in the prevention of protein precipitation and cataract formation in the eye, alpha crystallins have also been demonstrated in other tissues including the heart, brain, kidney, spleen and thymus. Mutation in alpha B crystallin is responsible for desmin-related myopathy and cataract 16 (Perng et al. 1999.). Alpha B crystallin is also a major component of the astrocytic inclusions known as "Rosenthal fibers" found in Alexander's disease (Iwaki et al. 1989). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
10% Glycerol - Immunogen
- Recombinant human alpha B crystallin (amino acids 1-175) purified from E. coli.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
1/1000 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- MCA2697 detects a band of approximately 20kDa in mouse eye cell lysates.
References for Alpha B Crystallin antibody
-
van Nierop, G.P. et al. (2015) Intrathecal CD8 T-cells of multiple sclerosis patients recognize lytic Epstein-Barr virus proteins.
Mult Scler. pii: 1352458515588581.
Further Reading
-
Perng, M.D. et al. (1999) The cardiomyopathy and lens cataract mutation in alphaB-crystallin alters its protein structure, chaperone activity, and interaction with intermediate filaments in vitro.
J Biol Chem. 274 (47): 33235-43.
- RRID
- AB_2228123
- UniProt
- P02511
- Entrez Gene
- CRYAB
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0006457 protein folding
- GO:0006936 muscle contraction
- GO:0005212 structural constituent of eye lens
- GO:0005634 nucleus
- GO:0006916 anti-apoptosis
- GO:0009408 response to heat
- GO:0032387 negative regulation of intracellular transport
- GO:0042803 protein homodimerization activity
- View More GO Terms
- GO:0051260 protein homooligomerization
MCA2697
152659
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