Aldehyde Dehydrogenase 3 antibody | OTI1B6

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Mouse anti Aldehyde Dehydrogenase 3

Aldehyde dehydrogenase 3 antibody recognizes aldehyde dehydrogenase isozyme 3, also known as aldehyde dehydrogenase type III, aldehyde dehydrogenase dimeric NADP-preferring or stomach aldehyde dehydrogenase. This antibody has been extensively validated for western blotting using whole cell lysates.

Aldehyde Dehydrogenase 3 Antibody with Control Lysate

Aldehyde dehydrogenase 3 antibody recognizes aldehyde dehydrogenase isozyme 3, also known as aldehyde dehydrogenase type III, aldehyde dehydrogenase dimeric NADP-preferring or stomach aldehyde dehydrogenase. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI1B6
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00254 WB 100 µl
VMA00254KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human aldehyde dehydrogenase 3 antibody recognizes the aldehyde dehydrogenase 3, also known as aldehyde dehydrogenase, dimeric NADP-preferring, aldehyde dehydrogenase isozyme 3, aldehyde dehydrogenase type III, aldehyde dehydrogenase, dimeric NADP-preferring and stomach aldehyde dehydrogenase.

Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by ALDH3A1 gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Sep 2008).

Mouse anti Human aldehyde dehydrogenase 3 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti aldehyde dehydrogenase 3 detects a band of approximately 50 kDa in A549 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human aldehyde dehydrogenase 3 (NP_000682) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg A549 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P30838 Related reagents
Entrez Gene
ALDH3A1 Related reagents
GO Terms
GO:0004029 aldehyde dehydrogenase (NAD) activity
GO:0005829 cytosol
GO:0005783 endoplasmic reticulum
GO:0006081 cellular aldehyde metabolic process
GO:0055114 oxidation-reduction process
GO:0008106 alcohol dehydrogenase (NADP+) activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Aldehyde Dehydrogenase 3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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