Aldehyde Dehydrogenase 2 antibody

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Mouse anti Aldehyde Dehydrogenase 2

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00235
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human aldehyde dehydrogenase 2 antibody recognizes aldehyde dehydrogenase, mitochondrial, also known as ALDH class 2, acetaldehyde dehydrogenase 2, aldehyde dehydrogenase, mitochondrial, liver mitochondrial ALDH and nucleus-encoded mitochondrial aldehyde dehydrogenase 2.

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. ALDH2 encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms (provided by RefSeq, Mar 2011).

Mouse anti Human aldehyde dehydrogenase 2 antibody detects a band of 56 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti aldehyde dehydrogenase 2 detects a band of approximately 56 kDa in HepG2 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant protein corresponding to amino acids 317-517 of human aldehyde dehydrogenase 2 expressed in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P05091 Related reagents
Entrez Gene
ALDH2 Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0042136 neurotransmitter biosynthetic process
GO:0004029 aldehyde dehydrogenase (NAD) activity
GO:0005759 mitochondrial matrix
GO:0005975 carbohydrate metabolic process
GO:0006069 ethanol oxidation
GO:0006805 xenobiotic metabolic process
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Aldehyde Dehydrogenase 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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