Aldehyde Dehydrogenase 2 antibody

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Mouse anti Aldehyde Dehydrogenase 2

Aldehyde dehydrogenase antibody recognizes aldehyde dehydrogenase, mitochondrial, also known as ALDH class 2, acetaldehyde dehydrogenase 2, liver mitochondrial ALDH or nucleus-encoded mitochondrial aldehyde dehydrogenase 2.

Aldehyde Dehydrogenase 2 Antibody with Control Lysate

Aldehyde dehydrogenase antibody recognizes aldehyde dehydrogenase, mitochondrial, also known as ALDH class 2, acetaldehyde dehydrogenase 2, liver mitochondrial ALDH or nucleus-encoded mitochondrial aldehyde dehydrogenase 2.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00235 WB 100 µl
VMA00235KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human aldehyde dehydrogenase 2 antibody recognizes aldehyde dehydrogenase, mitochondrial, also known as ALDH class 2, acetaldehyde dehydrogenase 2, aldehyde dehydrogenase, mitochondrial, liver mitochondrial ALDH and nucleus-encoded mitochondrial aldehyde dehydrogenase 2.

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. ALDH2 encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms (provided by RefSeq, Mar 2011).

Mouse anti Human aldehyde dehydrogenase 2 antibody detects a band of 56 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti aldehyde dehydrogenase 2 detects a band of approximately 56 kDa in HepG2 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant protein corresponding to amino acids 317-517 of human aldehyde dehydrogenase 2 expressed in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HepG2 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P05091 Related reagents
Entrez Gene
ALDH2 Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0042136 neurotransmitter biosynthetic process
GO:0004029 aldehyde dehydrogenase (NAD) activity
GO:0005759 mitochondrial matrix
GO:0005975 carbohydrate metabolic process
GO:0006069 ethanol oxidation
GO:0006805 xenobiotic metabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Aldehyde Dehydrogenase 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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