AKT3 antibody | I02/2H9

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Mouse anti AKT3

Product Type
PrecisionAb Monoclonal
Clone
I02/2H9
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00956
Datasheet
WB
SDS
100 µl loader

Mouse anti AKT3 antibody recognizes RAC-gamma serine/threonine-protein kinase, also known as PKB gamma. AKT is a serine/threonine kinase which regulates multiple cellular processes. Each of the three AKT isoforms shows a different tissue distribution, with AKT3 predominantly expressed in the brain (DuBois et al. 2019). The AKT isoforms also appear to have distinct functions, with AKT3 playing a critical role in the central nervous system (Xia et al. 2019). Deletion of AKT3 reduces brain size, indicating a role for AKT3 in regulation of central nervous system growth (Miao et al. 2016). Mutations in the gene encoding AKT3 have been associated with a range of developmental disorders of the brain, including megalencephaly (Alcantara et al. 2017). AKT3 overexpression has been identified in melanoma, and a tumor-promoting role for AKT3 has been found in colorectal cancer (Fang et al. 2018).

Product Details

Target Species
Human
Western Blotting
Mouse anti AKT3 detects a band of approximately 61 kDa in A172 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-479 of human AKT3
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q9Y243
Entrez Gene
AKT3
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0004674 protein serine/threonine kinase activity
GO:0007165 signal transduction
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005794 Golgi apparatus
GO:0006468 protein phosphorylation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of AKT3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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