AKT2 antibody

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Anti AKT2 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human AKT2 antibody (**VMA00091**)followed by detection with HRP conjugated Goat anti Rat IgG (1/10,000, STAR72) and visualized on the ChemiDoc MP with 60 second exposure. Arrow points to AKT2 (molecular weight 56 kDa).

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Rat anti AKT2

Product Type: PrecisionAb Monoclonal
Isotype: IgG2a
Format: Purified
Specificity: AKT2

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VMA00091 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rat anti Human AKT2 antibody recognizes AKT2, also known as protein kinase B beta, PKB beta and RAC-PK-beta.

AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phosphorylating several known proteins (provided by RefSeq, Jul 2008).

Rat anti Human AKT2 antibody detects a band of 56 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti AKT2 detects a band of approximately 56 kDa in HEK293 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Synthetic peptide corresponding to internal residues of human AKT2 protein
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rat IgG:HRP (Mouse Adsorbed)	STAR72	C E P	0.5 mg	Log in	Get Quote
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Description	Goat anti Rat IgG:HRP (Mouse Adsorbed)

UniProt: P31751
Entrez Gene: AKT2
GO Terms: GO:0005515 protein binding; GO:0005829 cytosol; GO:0004674 protein serine/threonine kinase activity; GO:0005524 ATP binding; GO:0005634 nucleus; GO:0008286 insulin receptor signaling pathway; GO:0010748 negative regulation of plasma membrane long-chain fatty acid transport; GO:0046326 positive regulation of glucose import; GO:0032000 positive regulation of fatty acid beta-oxidation; View More GO Terms; GO:0045725 positive regulation of glycogen biosynthetic process