AKT1 antibody | AbD30769

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Anti AKT1 Antibody, clone AbD30769 gallery image 1 — HepG2 lysate (25 μl) was loaded onto a Criterion TGX Stain-Free Precast Gel (lanes 2 - 5), run under reducing conditions and proteins were transferred onto a nitrocellulose membrane. Bio-Rad Precision Plus Protein Prestained Standards were run in lane 1. Human anti AKT1 (HCA308) was used to detect AKT1 (approximate MW of 65 kDa) in lanes 2 - 5. HRP-conjugated Human anti Bacterial Alkaline Phosphatase (HCA275P) was used as a secondary antibody at a dilution of 1/1000. The blot was visualized with the ChemiDoc MP Imaging System.

Human anti AKT1

Product Type: Monoclonal Antibody
Clone: AbD30769
Isotype: HuCAL Fab bivalent

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
HCA308	WB			0.1 mg

Summary
Secondary Antibodies

Human anti AKT1 antibody, clone AbD30769 recognizes AKT1, also known as RAC-alpha serine/threonine-protein kinase, protein kinase B alpha or Proto-oncogene c-Akt.
It belongs to the AGC kinase family (Brown and Banerji 2017). AKT activity is regulated downstream of receptor tyrosine kinases (RTKs) (Franke et al. 1995, Brown and Banerji 2017). Its substrates have a consensus phosphorylation motif: R-X-R-X-X-S/T-B (X is any amino acid and B denotes a preference for bulky hydrophobic residues) (Brown and Banerji 2017). A number of AKT substrates have been described including BAD (BCL2 associated agonist of cell death), FOXO (Forkhead Box), MDM2 (MDM2 oncogene), TSC2 (tuberous sclerosis 2), PRAS40 (proline rich Akt substrate), CDKN (cyclin-dependent kinase inhibitor) and GSK3β (glycogen synthase kinase beta) (Manning and Cantley 2007).
AKT is frequently activated in cancer (Manning and Toker 2017). In breast cancer, AKT isoforms play different roles during breast cancer progression (Riggio et al. 2017).

Product Details

Target Species: Human
Product Form: A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via an inactive variant of the bacterial alkaline phosphatase (BAP) fusion protein. The antibody is tagged with DYKDDDDK and His (HHHHHH) tags.
Preparation: Prepared by affinity chromatography
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.01% Thiomersal
Immunogen: Recombinant Human AKT1-GST fusion protein
Approx. Protein Concentrations: Antibody concentration 1.0 mg/ml

Storage Information

Storage: Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch

More Information

UniProt: P31749 Related reagents
Entrez Gene: AKT1 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0000060 protein import into nucleus, translocation; GO:0005829 cytosol; GO:0004674 protein serine/threonine kinase activity; GO:0005524 ATP binding; GO:0005547 phosphatidylinositol-3,4,5-trisphosphate binding; GO:0005654 nucleoplasm; GO:0005978 glycogen biosynthetic process; GO:0006417 regulation of translation; GO:0006469 negative regulation of protein kinase activity; GO:0006809 nitric oxide biosynthetic process; GO:0006924 activation-induced cell death of T cells; GO:0007186 G-protein coupled receptor protein signaling pathway; GO:0008286 insulin receptor signaling pathway; GO:0008629 induction of apoptosis by intracellular signals; GO:0008633 activation of pro-apoptotic gene products; GO:0042802 identical protein binding; GO:0009408 response to heat; GO:0010748 negative regulation of plasma membrane long-chain fatty acid transport; GO:0010975 regulation of neuron projection development; GO:0016071 mRNA metabolic process; GO:0046326 positive regulation of glucose import; GO:0046889 positive regulation of lipid biosynthetic process; GO:0019899 enzyme binding; GO:0018105 peptidyl-serine phosphorylation; GO:0048015 phosphatidylinositol-mediated signaling; GO:0030168 platelet activation; GO:0030235 nitric-oxide synthase regulator activity; GO:0030307 positive regulation of cell growth; GO:0031018 endocrine pancreas development; GO:0031295 T cell costimulation; GO:0031659 positive regulation of cyclin-dependent protein kinase activity involved in G1/S; GO:0031999 negative regulation of fatty acid beta-oxidation; GO:0034405 response to fluid shear stress; GO:0043325 phosphatidylinositol-3,4-bisphosphate binding; GO:0045429 positive regulation of nitric oxide biosynthetic process; GO:0045600 positive regulation of fat cell differentiation; GO:0045725 positive regulation of glycogen biosynthetic process; GO:0046777 protein autophosphorylation; GO:0048009 insulin-like growth factor receptor signaling pathway; GO:0048011 nerve growth factor receptor signaling pathway; GO:0051000 positive regulation of nitric-oxide synthase activity; GO:0070141 response to UV-A; GO:0090004 positive regulation of establishment of protein localization in plasma membrane
Acknowledgements: Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Regulatory: For research purposes only

Applications of AKT1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/200	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Human anti Bacterial Alkaline Phosphatase:HRP	HCA275P	0.1 mg	E WB

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