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AKT1 antibody | AbD30769

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Anti AKT1 Antibody, clone AbD30769 gallery image 1
HepG2 lysate (25 μl) was loaded onto a Criterion TGX Stain-Free Precast Gel (lanes 2 - 5), run under reducing conditions and proteins were transferred onto a nitrocellulose membrane. Bio-Rad Precision Plus Protein Prestained Standards were run in lane 1. Human anti AKT1 (HCA308) was used to detect AKT1 (approximate MW of 65 kDa) in lanes 2 - 5. HRP-conjugated Human anti Bacterial Alkaline Phosphatase (HCA275P) was used as a secondary antibody at a dilution of 1/1000. The blot was visualized with the ChemiDoc MP Imaging System.

Human anti AKT1

Product Type
Monoclonal Antibody
Clone
AbD30769
Isotype
HuCAL Fab bivalent
Product Code Applications Pack Size List Price Quantity
HCA308
Datasheet
WB
MSDS
0.1 mg loader

Human anti AKT1 antibody, clone AbD30769 recognizes AKT1, also known as RAC-alpha serine/threonine-protein kinase, protein kinase B alpha or Proto-oncogene c-Akt.
It belongs to the AGC kinase family (Brown and Banerji 2017). AKT activity is regulated downstream of receptor tyrosine kinases (RTKs) (Franke et al. 1995, Brown and Banerji 2017). Its substrates have a consensus phosphorylation motif: R-X-R-X-X-S/T-B (X is any amino acid and B denotes a preference for bulky hydrophobic residues) (Brown and Banerji 2017). A number of AKT substrates have been described including BAD (BCL2 associated agonist of cell death), FOXO (Forkhead Box), MDM2 (MDM2 oncogene), TSC2 (tuberous sclerosis 2), PRAS40 (proline rich Akt substrate), CDKN (cyclin-dependent kinase inhibitor) and GSK3β (glycogen synthase kinase beta) (Manning and Cantley 2007).
AKT is frequently activated in cancer (Manning and Toker 2017). In breast cancer, AKT isoforms play different roles during breast cancer progression (Riggio et al. 2017).

Product Details

Target Species
Human
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via an inactive variant of the bacterial alkaline phosphatase (BAP) fusion protein. The antibody is tagged with DYKDDDDK and His (HHHHHH) tags.
Preparation
Prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Recombinant Human AKT1-GST fusion protein
Approx. Protein Concentrations
Antibody concentration 1.0 mg/ml

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P31749 Related reagents
Entrez Gene
AKT1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000060 protein import into nucleus, translocation
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005547 phosphatidylinositol-3,4,5-trisphosphate binding
GO:0005654 nucleoplasm
GO:0005978 glycogen biosynthetic process
GO:0006417 regulation of translation
GO:0006469 negative regulation of protein kinase activity
GO:0006809 nitric oxide biosynthetic process
GO:0006924 activation-induced cell death of T cells
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0008286 insulin receptor signaling pathway
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0042802 identical protein binding
GO:0009408 response to heat
GO:0010748 negative regulation of plasma membrane long-chain fatty acid transport
GO:0010975 regulation of neuron projection development
GO:0016071 mRNA metabolic process
GO:0046326 positive regulation of glucose import
GO:0046889 positive regulation of lipid biosynthetic process
GO:0019899 enzyme binding
GO:0018105 peptidyl-serine phosphorylation
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030168 platelet activation
GO:0030235 nitric-oxide synthase regulator activity
GO:0030307 positive regulation of cell growth
GO:0031018 endocrine pancreas development
GO:0031295 T cell costimulation
GO:0031659 positive regulation of cyclin-dependent protein kinase activity involved in G1/S
GO:0031999 negative regulation of fatty acid beta-oxidation
GO:0034405 response to fluid shear stress
GO:0043325 phosphatidylinositol-3,4-bisphosphate binding
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045600 positive regulation of fat cell differentiation
GO:0045725 positive regulation of glycogen biosynthetic process
GO:0046777 protein autophosphorylation
GO:0048009 insulin-like growth factor receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051000 positive regulation of nitric-oxide synthase activity
GO:0070141 response to UV-A
GO:0090004 positive regulation of establishment of protein localization in plasma membrane
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of AKT1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/200 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Bacterial Alkaline Phosphatase:HRP HCA275P E WB 0.1 mg loader
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