AKR1C1 antibody

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Mouse anti AKR1C1

AKR1C1 antibody recognizes aldo-keto reductase family 1 member C1 also known as 20-alpha-hydroxysteroid dehydrogenase, chlordecone reductase homolog HAKRC, high-affinity hepatic bile acid-binding protein or HBAB.

AKR1C1 Antibody with Control Lysate

AKR1C1 antibody recognizes aldo-keto reductase family 1 member C1 also known as 20-alpha-hydroxysteroid dehydrogenase, chlordecone reductase homolog HAKRC, high-affinity hepatic bile acid-binding protein or HBAB.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00334 WB 100 µl
VMA00334KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human AKR1C1 antibody detects the aldo-keto reductase family 1 member C1, also known as 20-alpha-hydroxysteroid dehydrogenase (20-alpha-HSD), chlordecone reductase homolog HAKRC and high-affinity hepatic bile acid-binding protein (HBAB). Encoded by the AKR1C1 gene, aldo-keto reductase family 1 member C1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. Aldo-keto reductase family 1 member C1 catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. In the liver and intestine, it may have a role in the transport of bile, monitoring the intrahepatic bile acid concentration.

Mouse anti Human AKR1C1 antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human AKR1C1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q04828 Related reagents
Entrez Gene
AKR1C1 Related reagents
GO Terms
GO:0004033 aldo-keto reductase activity
GO:0005829 cytosol
GO:0055114 oxidation-reduction process
GO:0006805 xenobiotic metabolic process
GO:0046683 response to organophosphorus
GO:0008206 bile acid metabolic process
GO:0015721 bile acid and bile salt transport
GO:0030299 intestinal cholesterol absorption
GO:0032052 bile acid binding
GO:0042632 cholesterol homeostasis
GO:0051260 protein homooligomerization
GO:0047006 17-alpha,20-alpha-dihydroxypregn-4-en-3-one dehydrogenase activity
GO:0047042 androsterone dehydrogenase (B-specific) activity
GO:0047115 trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity
GO:0047718 indanol dehydrogenase activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of AKR1C1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti AKR1C1 detects a band of approximately 36 kDa in A549 cell lysates
Western Blotting
Anti AKR1C1 detects a band of approximately 36 kDa in A549 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB