AKR1C1 antibody

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Mouse anti AKR1C1

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00334
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human AKR1C1 antibody detects the aldo-keto reductase family 1 member C1, also known as 20-alpha-hydroxysteroid dehydrogenase (20-alpha-HSD), chlordecone reductase homolog HAKRC and high-affinity hepatic bile acid-binding protein (HBAB). Encoded by the AKR1C1 gene, aldo-keto reductase family 1 member C1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. Aldo-keto reductase family 1 member C1 catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. In the liver and intestine, it may have a role in the transport of bile, monitoring the intrahepatic bile acid concentration.

Mouse anti Human AKR1C1 antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti AKR1C1 detects a band of approximately 36 kDa in A549 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human AKR1C1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q04828 Related reagents
Entrez Gene
AKR1C1 Related reagents
GO Terms
GO:0004033 aldo-keto reductase activity
GO:0005829 cytosol
GO:0055114 oxidation-reduction process
GO:0006805 xenobiotic metabolic process
GO:0046683 response to organophosphorus
GO:0008206 bile acid metabolic process
GO:0015721 bile acid and bile salt transport
GO:0030299 intestinal cholesterol absorption
GO:0032052 bile acid binding
GO:0042632 cholesterol homeostasis
GO:0051260 protein homooligomerization
GO:0047006 17-alpha,20-alpha-dihydroxypregn-4-en-3-one dehydrogenase activity
GO:0047042 androsterone dehydrogenase (B-specific) activity
GO:0047115 trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity
GO:0047718 indanol dehydrogenase activity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of AKR1C1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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