AKR1A1 antibody | OTI9F1

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Mouse anti AKR1A1

AKR1A1 antibody recognizes alcohol dehydrogenase [NADP(+)], also known as HEL-S-165mP, alcohol dehydrogenase, aldehyde reductase, aldo-keto reductase family 1 member A1, dihydrodiol dehydrogenase 3, or epididymis secretory protein Li 6.

AKR1A1 Antibody with Control Lysate

AKR1A1 antibody recognizes alcohol dehydrogenase [NADP(+)], also known as HEL-S-165mP, alcohol dehydrogenase, aldehyde reductase, aldo-keto reductase family 1 member A1, dihydrodiol dehydrogenase 3, or epididymis secretory protein Li 6.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI9F1
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00109 WB 100 µl
VMA00109KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human AKR1A1 antibody recognizes AKR1A1, also known as HEL-S-165mP, alcohol dehydrogenase, alcohol dehydrogenase [NADP(+)], aldehyde reductase, aldo-keto reductase family 1 member A1, dihydrodiol dehydrogenase 3, epididymis secretory protein Li 6 and epididymis secretory sperm binding protein Li 165mP.

The AKR1A1 gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Multiple alternatively spliced transcript variants of AKR1A1 exist, all encoding the same protein (provided by RefSeq, Jan 2011).

Mouse anti Human AKR1A1 antibody detects a band of 38 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti AKR1A1 detects a band of approximately 38 kDa in LNCaP cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human AKR1A1 (NP_006057) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg LNCaP lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P14550 Related reagents
Entrez Gene
AKR1A1 Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0005515 protein binding
GO:0004032 aldehyde reductase activity
GO:0006006 glucose metabolic process
GO:0055114 oxidation-reduction process
GO:0008106 alcohol dehydrogenase (NADP+) activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of AKR1A1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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