AID antibody | EK2-5G9

Rat anti Human AID

Product Type
Monoclonal Antibody
Clone
EK2-5G9
Isotype
IgG2b
Specificity
AID

Product Code Applications Pack Size List Price Your Price Qty
MCA4756GT
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SDS Safety Datasheet SDS
E F * P * WB 50 µg loader
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loader
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Rat anti Human AID antibody, clone EK2-5G9 recognizes human Single-stranded DNA cytosine deaminase, also known as Activation-induced cytidine deaminase or Cytidine aminohydrolase. AID is a 198 amino acid ~24 kDa RNA-editing enzyme essential for the initiation of immunoglobulin class switch recombination, somatic hypermutation and gene conversion.

AID is selectively expressed by germinal centre B cells and B cell-derived lymphomas (Basso et al. 2012). Mutation in the AICDA gene is responsible for the autosomal recessive condition Immunodeficiency with hyper-IgM 2 (HIGM2) A rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE resulting in extreme susceptibility to bacterial infection.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 185-198 of human AID.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from Long Evans rats were fused with cells of the P3X63-Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1
Immunohistology - Paraffin 2
Western Blotting
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. EDTA pH8.0 is recommended for this purpose.>
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Germinal centre B cells

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Synonyms
ACTIVATION-INDUCED Cytidine Deaminase
RRID
AB_2224241
UniProt
Q9GZX7
Entrez Gene
AICDA
GO Terms
GO:0005515 protein binding
GO:0004126 cytidine deaminase activity
GO:0005737 cytoplasm
GO:0006397 mRNA processing
GO:0008270 zinc ion binding
GO:0030183 B cell differentiation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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