AID antibody | EK2-5G9
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Rat anti Human AID
- Product Type
- Monoclonal Antibody
- Clone
- EK2-5G9
- Isotype
- IgG2b
- Specificity
- AID
Antibody Quality and Performance Guarantee
| Rat anti Human AID antibody, clone EK2-5G9 recognizes human Single-stranded DNA cytosine deaminase, also known as Activation-induced cytidine deaminase or Cytidine aminohydrolase. AID is a 198 amino acid ~24 kDa RNA-editing enzyme essential for the initiation of immunoglobulin class switch recombination, somatic hypermutation and gene conversion. AID is selectively expressed by germinal centre B cells and B cell-derived lymphomas (Basso et al. 2012). Mutation in the AICDA gene is responsible for the autosomal recessive condition Immunodeficiency with hyper-IgM 2 (HIGM2) A rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE resulting in extreme susceptibility to bacterial infection. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to amino acids 185-198 of human AID.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from Long Evans rats were fused with cells of the P3X63-Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Flow Cytometry 1 | |
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| Immunohistology - Paraffin 2 | |
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| Western Blotting | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. EDTA pH8.0 is recommended for this purpose.>
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Germinal centre B cells
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2b Negative Control | MCA6006GA | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2b Negative Control | ||||||
- Synonyms
- ACTIVATION-INDUCED Cytidine Deaminase
- RRID
- AB_2224241
- UniProt
- Q9GZX7
- Entrez Gene
- AICDA
- GO Terms
- GO:0005515 protein binding
- GO:0004126 cytidine deaminase activity
- GO:0005737 cytoplasm
- GO:0006397 mRNA processing
- GO:0008270 zinc ion binding
- GO:0030183 B cell differentiation
MCA4756GT
140515
162099
162342
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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