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Adiponectin antibody | OTI7B11

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Western Blot Validated
Anti Adiponectin Antibody, clone OTI7B11 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with adiponectin antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 119 second exposure. Arrow points to adiponectin (molecular weight 27 kDa)

Mouse anti Adiponectin

Product Type
PrecisionAb Monoclonal
Clone
OTI7B11
Isotype
IgG2b
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00653
Datasheet
WB
SDS
100 µl loader

Mouse anti Human adiponectin antibody recognizes adiponectin, also known as 30 kDa adipocyte complement-related protein, adipose specific collagen-like factor and gelatin-binding protein 28.

ADIPOQ is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in ADIPOQ are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Apr 2010).

Mouse anti Human adiponectin antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti adiponectin antibody detects a band of approximately 27 kDa in C2C12 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
HEK293-derived recombinant human adiponectin (aa 19-244)

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q15848
Entrez Gene
ADIPOQ
GO Terms
GO:0005125 cytokine activity
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0006006 glucose metabolic process
GO:0006091 generation of precursor metabolites and energy
GO:0006635 fatty acid beta-oxidation
GO:0009749 response to glucose stimulus
GO:0010739 positive regulation of protein kinase A signaling cascade
GO:0010745 negative regulation of macrophage derived foam cell differentiation
GO:0010804 negative regulation of tumor necrosis factor-mediated signaling pathway
GO:0010875 positive regulation of cholesterol efflux
GO:0046326 positive regulation of glucose import
GO:0030853 negative regulation of granulocyte differentiation
GO:0032720 negative regulation of tumor necrosis factor production
GO:0033034 positive regulation of myeloid cell apoptosis
GO:0034115 negative regulation of heterotypic cell-cell adhesion
GO:0034383 low-density lipoprotein particle clearance
GO:0034612 response to tumor necrosis factor
GO:0042593 glucose homeostasis
GO:0042803 protein homodimerization activity
GO:0043124 negative regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043499 eukaryotic cell surface binding
GO:0045599 negative regulation of fat cell differentiation
GO:0045650 negative regulation of macrophage differentiation
GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process
GO:0045721 negative regulation of gluconeogenesis
GO:0045923 positive regulation of fatty acid metabolic process
GO:0050728 negative regulation of inflammatory response
GO:0050765 negative regulation of phagocytosis
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Adiponectin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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