Adiponectin antibody | OTI7B11

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Anti Adiponectin Antibody, clone OTI7B11 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with adiponectin antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 119 second exposure. Arrow points to adiponectin (molecular weight 27 kDa)

Mouse anti Adiponectin

Adiponectin antibody recognizes adiponectin, also known as 30 kDa adipocyte complement-related protein, adipose specific collagen-like factor or gelatin-binding protein 28.

Product Type: PrecisionAb™ Monoclonal
Clone: OTI7B11
Isotype: IgG2b
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00653 Datasheet	WB MSDS	100 µl	Log in		Get Quote

Summary
Secondary Antibodies

Mouse anti Human adiponectin antibody recognizes adiponectin, also known as 30 kDa adipocyte complement-related protein, adipose specific collagen-like factor and gelatin-binding protein 28.

ADIPOQ is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in ADIPOQ are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Apr 2010).

Mouse anti Human adiponectin antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Mouse anti adiponectin antibody detects a band of approximately 27 kDa in C2C12 cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from ascites

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
<50% Glycerol

Immunogen

HEK293-derived recombinant human adiponectin (aa 19-244)

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles
Guarantee: As supplied, 12 months from date of despatch

More Information

UniProt: Q15848 Related reagents
Entrez Gene: ADIPOQ Related reagents
GO Terms: GO:0005125 cytokine activity; GO:0005179 hormone activity; GO:0005615 extracellular space; GO:0006006 glucose metabolic process; GO:0006091 generation of precursor metabolites and energy; GO:0006635 fatty acid beta-oxidation; GO:0009749 response to glucose stimulus; GO:0010739 positive regulation of protein kinase A signaling cascade; GO:0010745 negative regulation of macrophage derived foam cell differentiation; GO:0010804 negative regulation of tumor necrosis factor-mediated signaling pathway; GO:0010875 positive regulation of cholesterol efflux; GO:0046326 positive regulation of glucose import; GO:0030853 negative regulation of granulocyte differentiation; GO:0032720 negative regulation of tumor necrosis factor production; GO:0033034 positive regulation of myeloid cell apoptosis; GO:0034115 negative regulation of heterotypic cell-cell adhesion; GO:0034383 low-density lipoprotein particle clearance; GO:0034612 response to tumor necrosis factor; GO:0042593 glucose homeostasis; GO:0042803 protein homodimerization activity; GO:0043124 negative regulation of I-kappaB kinase/NF-kappaB cascade; GO:0043499 eukaryotic cell surface binding; GO:0045599 negative regulation of fat cell differentiation; GO:0045650 negative regulation of macrophage differentiation; GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process; GO:0045721 negative regulation of gluconeogenesis; GO:0045923 positive regulation of fatty acid metabolic process; GO:0050728 negative regulation of inflammatory response; GO:0050765 negative regulation of phagocytosis
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of Adiponectin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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